Increased blood clearance rate of indium-111 oxine-labeled autologous CD4+ blood cells in untreated patients with Hodgkin's disease

Journal Article · Wed Aug 01 04:00:00 EDT 1990 · Blood (Journal of Hematology); (USA)

OSTI ID:6525752

Grimfors, G; Holm, G; Mellstedt, H; Schnell, P O; Tullgren, O; Bjoerkholm, M ^[1]

---

1. Karolinska Hospital, Stockholm (Sweden)

Untreated patients with Hodgkin's disease (HD) have a blood T-lymphocytopenia mainly caused by a reduction of the CD4+ subset. Indirect support for a sequestration of T cells in the spleen and tumor-involved lymphoid tissue has accumulated. To test the hypothesis that the blood CD4 T-lymphocytopenia in patients with HD is caused by an altered lymphocyte traffic, 12 untreated HD patients and five in complete clinical remission (CCR) were studied. Blood lymphocytes were collected by leukapheresis and gradient centrifugation, and were further purified by an adherence step. The cells were labeled with indium-111 oxine and reinfused intravenously into the patient. The radioactivity of CD4+ and CD8+ blood lymphocytes separated by immunoabsorption was measured from serial blood samples. CD4+ cells were eliminated more rapidly in untreated patients than patients in CCR. Repeated gamma camera imaging after autotransfusion of indium-111 oxine labeled cells demonstrated an accumulation of radioactivity in tumor-involved tissue of untreated patients. These findings support the concept of an enhanced elimination of CD4+ cells in patients with active HD that may contribute to the observed blood T-lymphocytopenia and may reflect a biologic response to the tumor.

• OSTI ID: 6525752
• Journal Information: Blood (Journal of Hematology); (USA), Journal Name: Blood (Journal of Hematology); (USA) Vol. 76:3; ISSN BLOOA; ISSN 0006-4971
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ADHESION
ANIMAL CELLS
AROMATICS
AZAARENES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD-PLASMA CLEARANCE
BODY FLUIDS
CAMERAS
CENTRIFUGATION
CLEARANCE
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
FRACTIONATION
GAMMA CAMERAS
HETEROCYCLIC COMPOUNDS
HODGKINS DISEASE
HYDROXY COMPOUNDS
IMAGE PROCESSING
IMMUNE SYSTEM DISEASES
INDIUM 111
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
LYMPHOMAS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXINE
PATHOGENESIS
PATIENTS
PROCESSING
PYRIDINES
QUINOLINES
RADIOISOTOPES
SEPARATION PROCESSES
SOMATIC CELLS