Labeling autologous leukocytes with indium-111 oxine
A method is described for labeling autologous leukocytes with indium-111 oxine, which is used for localizing inflammatory processes. To an aqueous solution of indium-111 chloride was added sodium acetate buffer, pH 5.0, and then a freshly prepared solution of oxine (8-hydroxyquinoline) in ethanol. After 3-5 minutes of incubation at room temperature, the chelated indium-111 oxine was extracted with chloroform, evaporated to dryness, redissolved in ethanol, and diluted with 0.9% saline. Leukocytes were isolated from venous blood by centrifugation at 300-350 g and resuspended in 0.9% saline. The cells were labeled by adding the indium-111 oxine solution to the leukocyte suspension. Clinical results have shown indium-111-labeled leukocytes to be highly specific for abscess localization. Indium-111 is useful for imaging because its gamma emissions are suitable for external detection and its half-life of 67 hours allows studies to be performed over several days without presenting an excessive radiation dose to the patient.
- Research Organization:
- Univ. of Utah, Salt Lake City
- OSTI ID:
- 5586866
- Journal Information:
- Am. J. Hosp. Pharm.; (United States), Journal Name: Am. J. Hosp. Pharm.; (United States) Vol. 37:6; ISSN AJHPA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
ABSCESSES
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DIAGNOSIS
ELECTRON CAPTURE RADIOISOTOPES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDIUM 111
INDIUM ISOTOPES
INFLAMMATION
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LEUKOCYTES
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXINE
PATHOLOGICAL CHANGES
PATIENTS
PYRIDINES
QUINOLINES
RADIOISOTOPES
SYMPTOMS
TRACER TECHNIQUES