Bronchial hyperreactivity occurs in steroid-treated guinea pigs depleted of leukocytes by cyclophosphamide

Journal Article · Wed May 01 04:00:00 EDT 1985 · J. Appl. Physiol.; (United States)

OSTI ID:6525031

Murlas, C; Roum, J H

The effects of cyclophosphamide and cortisone acetate treatment on O/sub 3/-induced changes in airway mucosal morphology and bronchial reactivity were assessed in guinea pigs. Animals in groups of four were studied at 2 or 6 h after O/sub 3/ (3.0 ppm, 2 h) and in one control group. Reactivity was determined by measuring specific airway resistance during intravenous acetylcholine infusion in intact, unanesthetized, spontaneously breathing animals. After testing, tracheal tissue was obtained from all animals for light microscopic examination. Another group of 10 drug-treated and 10 normal animals were tested at 2 h, 6 h, 1 day, and 4 days after O/sub 3/. Drug treatment resulted in substantial decreases in both circulating and airway mucosal granulocytes. Two hours after O/sub 3/, a marked decrease in airway mucosal goblet cells as well as ciliated cell damage occurred in both normal and treated animals. However, only in normal animals did neutrophilic infiltration develop thereafter. Nonetheless, hyperreactivity postozone occurred and progressed similarly in both groups. Our results indicate that acute O/sub 3/-induced bronchial hyperreactivity at 2 h is associated with signs of airway mucosal injury but appears independent of granulocyte changes. Airway neutrophilic infiltration and eosinophil depletion seem to be consequences of mucosal injury from O/sub 3/ and not causes of the bronchial hyperreactivity that results.

• OSTI ID: 6525031
• Journal Information: J. Appl. Physiol.; (United States), Journal Name: J. Appl. Physiol.; (United States) Vol. 5; ISSN JAPYA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETYLCHOLINE
ADRENAL HORMONES
AIR POLLUTION
AMIDES
AMINES
AMMONIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL EFFECTS
BRONCHI
CORTICOSTEROIDS
CORTISONE
DISEASES
DRUGS
ESTERS
GLUCOCORTICOIDS
GUINEA PIGS
HEMIC DISEASES
HORMONES
HYDROXY COMPOUNDS
KETONES
LEUKOPENIA
MAMMALS
MORPHOLOGICAL CHANGES
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OZONE
PARASYMPATHOMIMETICS
POLLUTION
PREGNANES
QUATERNARY COMPOUNDS
RESPIRATORY SYSTEM
RODENTS
STEROID HORMONES
STEROIDS
TOXICITY
VERTEBRATES