Ozone-induced bronchial hyperreactivity in guinea pigs is abolished by BW 755C or FPL 55712 but not by indomethacin

Journal Article · Fri Nov 01 04:00:00 EST 1985 · Am. Rev. Respir. Dis.; (United States)

OSTI ID:6185942

Lee, H K; Murlas, C

The authors investigated the effects of BW 755C, an inhibitor of both the cyclooxygenase and lipoxygenase pathways of arachidonic acid metabolism; FPL 55712, a selective antagonist of slow-reacting substance of anaphylaxis; and indomethacin, a cyclooxygenase inhibitor, on bronchial reactivity after ozone exposure. Guinea pigs in groups of 5 were treated with BW 755C, FPL 55712, or indomethacin and studied before and 30 min after a 15-min exposure to 3.0 ppm ozone. These animals were compared with a similarly exposed group that was untreated. Reactivity was determined by measuring specific airway resistance (SRaw) upon intravenous acetylcholine infusion in unanesthetized, spontaneously breathing animals. Prior to ozone exposure, they found that drug treatment did not affect either SRaw or muscarinic reactivity. After exposure to 3.0 ppm, all untreated guinea pigs showed substantial muscarinic hyperreactivity. Indomethacin treatment did not inhibit this effect. Furthermore, in the indomethacin-treated animals, marked elevations in SRaw after ozone occurred. In contrast, no change in SRaw or muscarinic reactivity occurred after ozone in any animal treated with either BW 755C or FPL 55712. The authors conclude that ozone-induced bronchial hyperreactivity in the guinea pig rapidly develops after a brief, high-level exposure. This effect may be mediated in part by lipoxygenase products derived from lung arachidonic acid metabolism post-ozone period.

• Research Organization: Univ. of Cincinnati School of Medicine, OH
• OSTI ID: 6185942
• Journal Information: Am. Rev. Respir. Dis.; (United States), Journal Name: Am. Rev. Respir. Dis.; (United States) Vol. 5; ISSN ARRDA
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETYLCHOLINE
AMINES
AMMONIUM COMPOUNDS
ANIMALS
ARACHIDONIC ACID
AUTONOMIC NERVOUS SYSTEM AGENTS
AZOLES
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BRONCHI
CARBOXYLIC ACIDS
CHEMISTRY
DRUGS
ENZYME INHIBITORS
ENZYMES
ESTERS
GUINEA PIGS
HETEROCYCLIC COMPOUNDS
MAMMALS
METABOLISM
MONOCARBOXYLIC ACIDS
NEUROREGULATORS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
OZONE
PARASYMPATHOMIMETICS
PYRAZOLES
QUATERNARY COMPOUNDS
RESPIRATORY SYSTEM
RODENTS
VERTEBRATES