Development of a chemically defined serum-free medium for differentiation of rat adipose precursor cells

Journal Article · Thu Jan 01 04:00:00 EST 1987 · Exp. Cell Res.; (United States)

DOI:https://doi.org/10.1016/0014-4827(87)90412-5· OSTI ID:6523930

Deslex, S; Negrel, R; Ailhaud, G

Stromal-vascular cells from the epididymal fat pad of 4-week-old rats, when cultured in a medium containing insulin or insulin-like growth factor, IFG-I, triiodothyronine and transferrin, were able to undergo adipose conversion. Over ninety percent of the cells accumulated lipid droplets and this proportion was reduced in serum-supplemented medium. The adipose conversion was assessed by the development of lipoprotein lipase (LPL) and glycerol-3-phosphate dehydrogenase (GPDH) activities, (/sup 14/)glucose incorporation into polar and neutral lipids, triacylglycerol accumulation and lipolysis in response to isoproterenol. Similar results were obtained with stromal-vascular cells from rat subcutaneous and retroperitoneal adipose tissues. Stromal-vascular cells required no adipogenic factors in addition to the components of the serum-free medium. Insulin was required within a physiological range of concentrations for the emergence of LPL and at higher concentrations for that of GPDH. When present at concentrations ranging from 2 to 50 nM, IGF-I was able to replace insulin for the expression of both LPL and and GPDH. The development of a serum free, chemically defined medium for the differentiation of diploid adiopose precursor cells opens up the possibility of characterizing inhibitors or activators of the adipose conversion process.

• Research Organization: Centre de Biochimie du CNRS, Nice, France
• OSTI ID: 6523930
• Journal Information: Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 168:1; ISSN ECREA
• Country of Publication: United States
• Language: English

Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ALDEHYDES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BODY
CARBOHYDRATES
CARBON 14 COMPOUNDS
CELL DIFFERENTIATION
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
CULTURE MEDIA
ENZYMES
EVALUATION
FAT CELLS
GLOBULINS
GLOBULINS-BETA
GLUCOSE
HEXOSES
HORMONES
INSULIN
LABELLED COMPOUNDS
MAMMALS
METALLOPROTEINS
MONOSACCHARIDES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEPTIDE HORMONES
PROTEINS
RATS
RODENTS
SACCHARIDES
SOMATIC CELLS
THYROID HORMONES
TISSUES
TRANSFERRIN
TRIIODOTHYRONINE
UPTAKE
VERTEBRATES