Changes in adipose tissue stromal-vascular cells in primary culture due to porcine sera

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5461683

Jewell, D E; Hausman, G J

This study was conducted to determine the response of rat stromal-vascular cells to pig sea. Sera were collected from unselected contemporary (lean) and high backfat thickness selected (obese) pigs. Sera from obese pigs were collected either by exsanguination or cannulation. sera from lean pigs during the growing phase (45 kg) and the fattening phase (100-110 kg) were collected. Stromal-vascular cells derived rom rat inguinal tissue were cultured on either 25 cm/sup 2/ flasks, collagen-coated coverslips or petri dishes. Cell proliferation was measured by (/sup 3/H)-thymidine incorporation during the fourth day of culture. Coverslip cultures were used for histochemical analysis. Petri dish cultures were used for analysis of Sn-glycerol-3-phosphate dehydrogenase (GPDH) activity. All cells were plated for 24 hours in media containing 10 fetal bovine sera. Test media contained 2.5, 5.0, 10.0% sera. Sera from obese pigs increased GPDH activity and fat cell production when compared to the lean controls. The increased concentration of sera increased esterase activity and lipid as measured with oil red O. The sera from obese pigs collected at slaughter stimulated more fat cell production than obese sera collected by cannulation. These studies show there are adipogenic factors in obese pigs sera which promote fat cell development in primary cell culture.

• Research Organization: Dept. of Agriculture - ARS Russel Research Laboratory, Athens, GA
• OSTI ID: 5461683
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AZINES
BODY
CELL CULTURES
CELL PROLIFERATION
CONNECTIVE TISSUE
DOMESTIC ANIMALS
ENZYMES
ESTERASES
HETEROCYCLIC COMPOUNDS
HYDROLASES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PYRIMIDINES
RATS
RIBOSIDES
RODENTS
SWINE
THYMIDINE
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES