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5. cap alpha. -reductase activity in rat adipose tissue

Journal Article · · Proc. Soc. Exp. Biol. Med.; (United States)
; ;

We measured the 5 ..cap alpha..-reductase activity in isolated cell preparations of rat adipose tissue using the formation of (/sup 3/H) dihydrotestosterone from (/sup 3/H) testosterone as an endpoint. Stromal cells were prepared from the epididymal fat pad, perinephric fat, and subcutaneous fat of male rats and from perinephric fat of female rats. Adipocytes were prepared from the epididymal fat pad and perinephric fat of male rats. Stromal cells from the epididymal fat pad and perinephric fat contained greater 5..cap alpha..-reductase activity than did the adipocytes from these depots. Stromal cells from the epididymal fat pad contained greater activity than those from perinephric and subcutaneous depots. Perinephric stromal cells from female rats were slightly more active than those from male rats. Estradiol (10/sup -8/ M), when added to the medium, caused a 90% decrease in 5..cap alpha..-reductase activity. Aromatase activity was minimal, several orders of magnitude less than 5..cap alpha..-reductase activity in each tissue studied.

Research Organization:
Univ. of Massachusetts Medical School, Worcester (USA)
OSTI ID:
5410003
Journal Information:
Proc. Soc. Exp. Biol. Med.; (United States), Journal Name: Proc. Soc. Exp. Biol. Med.; (United States) Vol. 186:2; ISSN PSEBA
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ANDROGENS
ANDROSTANES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOSYNTHESIS
BODY
CONNECTIVE TISSUE
CONNECTIVE TISSUE CELLS
ENZYME ACTIVITY
ENZYMES
ESTRADIOL
ESTRANES
ESTROGENS
FAT CELLS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
MAMMALS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
RATS
RODENTS
SOMATIC CELLS
STEROID HORMONES
STEROIDS
SYNTHESIS
TESTOSTERONE
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES