Inhibition of epidermal growth factor receptor biosynthesis caused by the src oncogene product, pp60 sup v- src

Journal Article · Thu Mar 01 04:00:00 EST 1990 · Molecular and Cellular Biology; (USA)

DOI:https://doi.org/10.1128/MCB.10.3.1254· OSTI ID:6511891

Wasilenko, W J ^[1]; Nori, M; Testerman, N; Weber, M J ^[2]

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1. Dept. of Microbiology and Immunology, Eastern Virginia Medical School, Norfolk, VA (US)
2. Dept. of Microbiology and Cancer Center, Univ. of Virginia Health Sciences Center, Charlottesville, VA (US)

The authors have previously shown that an intracellular mechanism down regulates epidermal growth factor (EGF) receptor levels in rodent fibroblasts transformed by the {ital src} oncogene. They now report that this down regulation is due to an inhibition of EGF receptor biosynthesis. With Rat-1 (R1) cell infected with a temperature-sensitive {ital src} mutant, we found that {sup 125}I-labeled EGF binding to cells began to decrease soon after the activation of pp60{sup v-{ital src}} by shift down to the permissive temperature for transformation. This effect of {ital src} on EGF receptors was reversible. Pulse-chase studies with ({sup 35}S)methionine-labeled cells revealed that the tyrosine protein kinase activity of pp60{sup v-{ital src}} had little if any effect on EGF receptor degradation rate. By contrast, the expression of pp60{sup v-{ital src}} caused a large reduction in the apparent rate of EGF receptor biosynthesis.

• OSTI ID: 6511891
• Journal Information: Molecular and Cellular Biology; (USA), Journal Name: Molecular and Cellular Biology; (USA) Vol. 10:3; ISSN MCEBD; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL FUNCTIONS
BIOLOGICAL PATHWAYS
CARBOXYLIC ACIDS
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYME INDUCTION
ENZYMES
EVEN-ODD NUCLEI
FIBROBLASTS
FUNCTIONS
GENE REGULATION
GENE REPRESSORS
GENES
GROWTH FACTORS
HYDROXY ACIDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MEMBRANE PROTEINS
MITOGENS
MOLECULAR BIOLOGY
NUCLEI
NUCLEOPROTEINS
ODD-EVEN NUCLEI
ONCOGENES
ONCOGENIC TRANSFORMATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
RADIOISOTOPES
RATS
RECEPTORS
RODENTS
SOMATIC CELLS
SULFUR 35
SULFUR ISOTOPES
TRACER TECHNIQUES
TRANSFERASES
TYROSINE
VERTEBRATES