Characterization of pp85; A target of oncogenes and growth factor receptors
Journal Article
·
· Molecular and Cellular Biology; (USA)
- Dept. of Biochemistry, Tufts Univ., Health Sciences Campus, Boston, MA (US)
- Dept. of Pathology, Harvard Medical School, Boston, MA (US)
An 85,000-molecular-weight polypeptide (85 K polypeptide) has previously been identified as a common substrate for tyrosine phosphorylation upon polyomavirus middle T transformation or upon platelet-derived growth factor stimulation of 3T3 cells. In each case, pp85 has an associated phosphatidylinositol kinase activity.The tissue distribution of pp85 was determined by middle T blotting experiments; the highest levels were found in brain, lung, and spleen tissues. High-resolution examination of 85 K by isoelectric focusing demonstrated that there are at least 10 different forms. These were resolved into two families, 85 K and 86 K; the ratio of the two families changed in different cells. Similar forms were found for pp85 associated with pp60{sup v-{ital src}}. Individual species within each family differed by phosphorylation. This paper reports that analysis of pp85 and pp86 by immunoprecipitation with anti-phosphotyrosine antibody showed increasing phosphorylation in response to middle T or pp60{sup v-{ital src}} transformation.
- OSTI ID:
- 6511615
- Journal Information:
- Molecular and Cellular Biology; (USA), Journal Name: Molecular and Cellular Biology; (USA) Vol. 10:6; ISSN MCEBD; ISSN 0270-7306
- Country of Publication:
- United States
- Language:
- English
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Tue May 01 00:00:00 EDT 1990
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
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·
OSTI ID:6600483
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OSTI ID:5202435
Related Subjects
550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANTIGEN-ANTIBODY REACTIONS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL PATHWAYS
BODY
BRAIN
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL TRANSFORMATIONS
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
ENZYME ACTIVITY
ENZYMES
GENES
HYDROXY ACIDS
INOSITOLS
KINETICS
LUNGS
MEMBRANE PROTEINS
MONOSACCHARIDES
NERVOUS SYSTEM
ONCOGENES
ONCOGENIC TRANSFORMATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
POLYPEPTIDES
PROTEINS
REACTION KINETICS
RECEPTORS
RESPIRATORY SYSTEM
SACCHARIDES
SPECIFICITY
SPLEEN
TISSUES
TRANSFERASES
TYROSINE
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANTIGEN-ANTIBODY REACTIONS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL PATHWAYS
BODY
BRAIN
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL TRANSFORMATIONS
CENTRAL NERVOUS SYSTEM
CHEMICAL REACTIONS
ENZYME ACTIVITY
ENZYMES
GENES
HYDROXY ACIDS
INOSITOLS
KINETICS
LUNGS
MEMBRANE PROTEINS
MONOSACCHARIDES
NERVOUS SYSTEM
ONCOGENES
ONCOGENIC TRANSFORMATIONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
POLYPEPTIDES
PROTEINS
REACTION KINETICS
RECEPTORS
RESPIRATORY SYSTEM
SACCHARIDES
SPECIFICITY
SPLEEN
TISSUES
TRANSFERASES
TYROSINE