Interaction between human peripheral blood monocytes and tumor promoters: Effect on growth differentiation and function in vitro

Journal Article · Wed Aug 01 04:00:00 EDT 1990 · Journal of Biological Response Modifiers; (USA)

OSTI ID:6508122

Keisari, Y; Bucana, C; Markovich, S; Campbell, D E ^[1]

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1. Tel Aviv Univ. (Israel)

Studies on the differentiation and activation of human monocytes in tissue cultures have usually been limited by the deterioration of human monocytes and macrophages in long-term cultures. In this study, we attempted to establish long-term human monocyte/macrophage cultures using the phorbol ester 12-0 tetradecanoyl-phorbol-13-acetate (TPA), and we studied the morphology, function, and biochemical properties of such treated human blood monocytes. Enriched suspensions of monocytes were obtained using Ficoll-Hypaque gradient and cultured in the absence or presence of various concentrations of TPA. Samples were removed at different times and processed for scanning electron microscopy. Parallel samples were examined for numbers of adherent cells, phagocytosis, oxidative burst, beta-galactosidase assays, and lectin-mediated erythrolysis. TPA-treated monocytes survived in larger numbers in culture for up to 7 weeks and were more pleomorphic and exhibited higher beta-galactosidase activities after 14 days in culture than untreated monocytes. TPA-treated cells and untreated cells in long-term cultures showed a decrease in their oxidative burst activity while their phagocytic activity was not affected, and the TPA treatment augmented the lysis of wheat germ agglutinin-opsonized erythrocytes by the cultured monocytes. TPA treatment of adherent human monocytes resulted in cell cultures with increased numbers of viable and functionally adherent cells for extended periods of time and does not seem to interfere with the differentiation and maturation of the cells in culture.

• OSTI ID: 6508122
• Journal Information: Journal of Biological Response Modifiers; (USA), Journal Name: Journal of Biological Response Modifiers; (USA) Vol. 9:4; ISSN 0732-6580; ISSN JBRMD
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADHESION
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CELL DIFFERENTIATION
CHEMICAL PROPERTIES
CONNECTIVE TISSUE CELLS
ELECTRON MICROSCOPY
ENZYME ACTIVITY
ENZYMES
ERYTHROCYTES
ESTERS
GALACTOSIDASE
GLYCOSYL HYDROLASES
HYDROLASES
IN VITRO
LEUKOCYTES
LYSIS
MACROPHAGES
MAMMALS
MAN
MATERIALS
MICROSCOPY
MONOCYTES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PHAGOCYTES
PHAGOCYTOSIS
PHORBOL ESTERS
PRIMATES
SCANNING ELECTRON MICROSCOPY
SOMATIC CELLS
VERTEBRATES