Nylon wool adherent accessory cell can augment CTL to MOPC 104E in the presence of 12-O-tetradecanoyl-phorbol-13-acetate (TPA)

Conference · Sat Mar 01 04:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5415115

Miura, T; Ghanta, V K; Hiramoto, R

An active tumor-promoting agent, 12-O-tetradecanoyl-phorbol-13-acetate (TPA) is effective in generating cytotoxic lymphocytes toward the weakly antigenic tumor, MOPC 104E plasmacytoma. Enhanced stimulation of non-adherent spleen lymphocytes (NSC), from which plastic adherent cells were removed, was observed at a concentration of 2 and 10 ng/ml of TPA. This enhancing effect of TPA was obtained at the NSC responder:MOPC 104E stimulator cell ratios of 10:1 and 100:1. T-cells enriched by passage through nylon wool columns from normal or in vivo primed spleens were not responsive to TPA, even after reconstitution with plastic adherent cells. These observations suggest that the generation of cytotoxic lymphocytes by TPA is augmented by an accessory cell present in NSC, which is effectively removed by passage through nylon wool column. The nylon wool adherent accessory cell differs from the plastic adherent macrophages in that the plastic adherent cells were not able to augment the activity of T-cells with TPA.

• Research Organization: Gunma Univ., Japan
• OSTI ID: 5415115
• Report Number(s): CONF-8604222-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
• Country of Publication: United States
• Language: English

Related Subjects

560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADHESION
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARCINOGENS
CONNECTIVE TISSUE CELLS
DISEASES
ESTERS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
NEOPLASMS
ORGANIC COMPOUNDS
ORGANS
PHORBOL ESTERS
SOMATIC CELLS
SPLEEN