Monoclonal antibody to a subset of human monocytes found only in the peripheral blood and inflammatory tissues

Journal Article · Tue Jul 15 04:00:00 EDT 1986 · J. Immunol.; (United States)

OSTI ID:5388606

Zwadlo, G; Schlegel, R; Sorg, C

A monoclonal antibody is described that was generated by immunizing mice with cultured human blood monocytes. The antibody (27E10) belongs to the IgG1 subclass and detects a surface antigen at M/sub r/ 17,000 that is found on 20% of peripheral blood monocytes. The antigen is increasingly expressed upon culture of monocytes, reaching a maximum between days 2 and 3. Stimulation of monocytes with interferon-..gamma.. (IFN-..gamma..), 12-O-tetradecanoyl-phorbol-13-acetate (TPA), and lipopolysaccharide (LPS) Ylalanine (fMLP) increased the 27E10 antigen density. The amount of 27E10-positive cells is not or is only weakly affected. The antigen is absent from platelets, lymphotyces, and all tested human cell lines, yet it cross-reacts with 15% of freshly isolated granulocytes. By using the indirect immunoperoxidase technique, the antibody is found to be negative on cryostat sections of normal human tissue (skin, lung, and colon) and positive on only a few monocyte-like cells in liver and on part of the cells of the splenic red pulp. In inflammatory tissue, however, the antibody is positive on monocytes/macrophages and sometimes on endothelial cells and epidermal cells, depending on the stage and type of inflammation, e.g., BCG ranulomas are negative, whereas psoriasis vulgaris, atopic dermatitis, erythrodermia, pressure urticaria, and periodontitis contain positively staining cells. In contact eczemas at different times after elicitation (6 hr, 24 hr, and 72 hr), the 27E10 antigen is seen first after 24 hr on a few infiltrating monocytes/macrophages, which increase in numbers after 72 hr.

• Research Organization: Universitaets-Hautklinik, Muenster, West Germany
• OSTI ID: 5388606
• Journal Information: J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 137:2; ISSN JOIMA
• Country of Publication: United States
• Language: English

Related Subjects

551000 -- Physiological Systems
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIBODIES
ANTIBODY FORMATION
ANTIGEN-ANTIBODY REACTIONS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOHYDRATES
CARCINOGENS
DISEASES
ESTERS
GROWTH FACTORS
INFLAMMATION
INTERFERON
LEUKOCYTES
LIPIDS
LIPOPOLYSACCHARIDES
LYMPHOKINES
MAMMALS
MAN
MATERIALS
MITOGENS
MONOCLONAL ANTIBODIES
MONOCYTES
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PHORBOL ESTERS
POLYSACCHARIDES
PRIMATES
PROTEINS
SACCHARIDES
SKIN DISEASES
SYMPTOMS
TISSUES
VERTEBRATES