Two subgroups of T helper cells in F/sub 1/ hybrid mice revealed by negative selection to heterologous erythrocytes in vivo. [Gamma radiation]
Journal Article
·
· J. Immunol.; (United States)
OSTI ID:6503551
The specificity of heterozygous T cells for H-2-associated antigen in vivo was studied by inducing negative selection to sheep erythrocytes (SRC) in irradiated mice. Purified (CBA x C57BL/6)F/sub 1/ T cells plus SRC were transferred to irradiated F/sub 1/ or parental strain mice and then recovered from thoracic duct lymph 1 to 2 days later to measure T helper function. When F/sub 1/ T cells were filtered with SRC through irradiated F/sub 1/ mice the cells lost the capacity to stimulate anti-SRC responses by CBA, C57BL/6 or F/sub 1/ B cells. The unresponsiveness was specific (normal responses were found against horse erythrocytes) and there was no evidence that suppression was involved. By contrast, F/sub 1/ T cells filtered with SRC through irradiated mice of one parental strain failed to stimulate anti-SRC responses by B cells of this strain but retained the capacity to stimulate B cells of the opposite parental strain and F/sub 1/ B cells. Both primed and unprimed T cells gave similar results and subsequent positive selection of the T cells to SRC in irradiated F/sub 1/ mice for 5 days did not abrogate the restriction. The phenomenon was shown to be linked to the H-2 complex but not to Ig allotype.
- Research Organization:
- Univ. of Pennsylvania, Philadelphia
- OSTI ID:
- 6503551
- Journal Information:
- J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 121:5; ISSN JOIMA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOLOGY
BLOOD
BLOOD CELLS
BODY FLUIDS
ELECTROMAGNETIC RADIATION
ERYTHROCYTES
GAMMA RADIATION
GENETICS
HYBRIDIZATION
IMMUNE REACTIONS
IONIZING RADIATIONS
MAMMALS
MICE
RADIATION EFFECTS
RADIATIONS
RODENTS
SOMATIC CELLS
THYMOCYTES
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIGENS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOLOGY
BLOOD
BLOOD CELLS
BODY FLUIDS
ELECTROMAGNETIC RADIATION
ERYTHROCYTES
GAMMA RADIATION
GENETICS
HYBRIDIZATION
IMMUNE REACTIONS
IONIZING RADIATIONS
MAMMALS
MICE
RADIATION EFFECTS
RADIATIONS
RODENTS
SOMATIC CELLS
THYMOCYTES
VERTEBRATES