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Radiosensitization by diamide at low radiation doses

Conference · · Int. J. Radiat. Oncol., Biol. Phys.; (United States)
OSTI ID:6500370
In vitro studies in this laboratory indicated that at low radiation doses (0-3 Gy), sensitization by misonidazole diminishes. The authors low dose assay uses a partially automated microscopic analysis procedure which scores both surviving and killed cells. If enough cells are scored, this assay provides much better accuracy at low doses than the conventional assay. Recently, they applied their low dose assay to a study of sensitization by diamide. At high doses (D = 6.5 to 10 Gy, S = 0.1 to 0.01) an enhancement ratio (ER) of 2.6 for hypoxic cells and 1.1 for aerobic cells was obtained. At low doses, 0-2.5 Gy, diamide still sensitized hypoxic cells well; the ER at 1 Gy (S = 0.8) was 2.0. The fact that diamide shows good sensitization at low doses with this assay adds to the credibility of the assay and reduces the likelihood that these observations with misonidazole are an artifact of this system.
Research Organization:
British Columbia Cancer Research Centre, Vancouver
OSTI ID:
6500370
Report Number(s):
CONF-8311204-
Conference Information:
Journal Name: Int. J. Radiat. Oncol., Biol. Phys.; (United States) Journal Volume: 10:8
Country of Publication:
United States
Language:
English