Fluorescence line-narrowing spectrometry: Application to the study of benzo(a)pyrene metabolic pathways

Technical Report · Fri Jul 01 04:00:00 EDT 1988

OSTI ID:6500299

Zamzow, D S

The application of fluorescence line-narrowing spectrometry (FLNS) to the study of the pathways involved in the metabolic activation of the environmental contaminant and carcinogen benzo(a)pyrene is described. Fluorescence line-narrowed (FLN) spectra of benzo(a)pyrene, 6-methyl-benzo(a)pyrene, and a number of benzo(a)pyrene-nucleoside adducts are presented. The activation of benzo(a)pyrene (BP) to metabolites capable of binding to DNA in the in vitro horseradish peroxidase-catalyzed binding of BP to DNA, the DNA from mice exposed (in vivo) to BP, and the DNA from fish exposed (in vivo) to a number of polycyclic aromatic hydrocarbons, including BP, is investigated by the analysis of the FLN spectra obtained from these samples. 65 refs., 22 figs., 4 tabs.

• Research Organization: Ames Lab., IA (USA)
• DOE Contract Number: W-7405-ENG-82
• OSTI ID: 6500299
• Report Number(s): IS-T-1327; ON: DE89007609
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550500 -- Metabolism
59 BASIC BIOLOGICAL SCIENCES
ADDUCTS
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BIOCHEMICAL REACTION KINETICS
CONDENSED AROMATICS
DNA
DNA ADDUCTS
ENERGY LEVELS
ENZYMES
EXCITED STATES
FISHES
FLUORESCENCE
HYDROCARBONS
KINETICS
LINE NARROWING
LUMINESCENCE
MAMMALS
METABOLIC ACTIVATION
METABOLISM
METABOLITES
MICE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEROXIDASES
POLYCYCLIC AROMATIC HYDROCARBONS
PYRENE
REACTION KINETICS
RODENTS
SPECTRA
VERTEBRATES
VIBRATIONAL STATES