Ultraviolet B irradiation induces changes in the distribution and release of arachidonic acid, dihomo-gamma-linolenic acid, and eicosapentaenoic acid in human keratinocytes in culture
There is increasing evidence that derivatives of 20-carbon polyunsaturated fatty acids, the eicosanoids, play an important role in the inflammatory responses of the human skin. To better understand the metabolic fate of fatty acids in the skin, the effect of ultraviolet B (UVB) irradiation (280-320 nm) on the distribution and release of /sup 14/C-labeled arachidonic acid, dihomo-gamma-linolenic acid, and eicosapentaenoic acid in human keratinocytes in culture was investigated. Ultraviolet B irradiation induced the release of all three /sup 14/C-labeled fatty acids from the phospholipids, especially from phosphatidylethanolamine, and this was accompanied by increased labeling of the nonphosphorus lipids. This finding suggests that UVB induces a significant liberation of eicosanoid precursor fatty acids from cellular phospholipids, but the liberated fatty acids are largely reincorporated into the nonphosphorus lipids. In conclusion, the present study suggests that not only arachidonic acid but also dihomo-gamma-linolenic acid, and eicosapentaenoic acid might be involved in the UVB irradiation-induced inflammatory reactions of human skin.
- Research Organization:
- Univ. of Turku, Finland
- OSTI ID:
- 6498740
- Journal Information:
- J. Invest. Dermatol.; (United States), Journal Name: J. Invest. Dermatol.; (United States) Vol. 5; ISSN JIDEA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL CULTURES
DISTRIBUTION
EICOSANOIC ACID
ELECTROMAGNETIC RADIATION
EPIDERMIS
EPITHELIUM
INFLAMMATION
ISOTOPE APPLICATIONS
KERATIN
LABELLED COMPOUNDS
LINOLENIC ACID
LIPIDS
MAMMALS
MAN
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
PRIMATES
PROTEINS
RADIATION EFFECTS
RADIATIONS
SCLEROPROTEINS
SKIN
SYMPTOMS
TISSUE DISTRIBUTION
TISSUES
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
VERTEBRATES