Eicosapentaenoic and dihomo gamma linolenic acid metabolism by cultured rat mesangial cells

Journal Article · Sat Dec 31 23:00:00 EST 1988 · Am. J. Physiol.; (United States)

OSTI ID:6499558

Scharschmidt, L A; Gibbons, N B; Neuwirth, R

To better understand the effects of dietary fatty acid manipulations on glomerular function, we compared mesangial incorporation, release, and metabolism of arachidonic (AA), eicosapentaenoic (EPA), and dihomo gamma linolenic (DHG) acids. We found marked differences in mesangial handling of these fatty acids. AA was incorporated into lipids of mesangial cells much more rapidly than EPA or DHG. Ionophore-induced stimulation of fatty acid release from mesangial cells prelabeled with (/sup 14/C)AA, (/sup 14/C)EPA, or (/sup 14/C)DHG caused a release of labeled AA greater than DHG much less than EPA, respectively. Preloading mesangial cells with DHG or EPA for 24 h reduced subsequent basal, ionophore-, and hormone-stimulated prostaglandin E2 (PGE2) synthesis. Finally, unlike AA, neither EPA nor DHG was converted to a significant extent by mesangial cyclooxygenase or lipoxygenase. Thus the mesangial metabolism of DHG and EPA differs both quantitatively and qualitatively from that of AA. Furthermore, EPA and DHG inhibit metabolism of AA at the level of mesangial cyclooxygenase.

• Research Organization: Albert Einstein College of Medicine, Bronx, NY (USA)
• OSTI ID: 6499558
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 256; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARACHIDONIC ACID
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
COMPARATIVE EVALUATIONS
EICOSANOIC ACID
ENZYMES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LINOLENIC ACID
MAMMALS
METABOLISM
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGENASES
PROSTAGLANDINS
RATS
RODENTS
TRACER TECHNIQUES
VERTEBRATES