Physicochemical stability and inactivation of human and simian rotaviruses

Journal Article · Wed Apr 01 04:00:00 EST 1987 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6498130

Meng, Z D; Birch, C; Heath, R; Gust, I

The effects of various physical and chemical treatments on the stability of a human serotype 1 rotavirus and simian agent 11 (SA11) were compared by using a fluorescence focus assay. The infectivity of both strains was retained after storage at room temperature for 14 days, 4 degree C for 22 days, and -20 degree C for 32 days; lyophilization; and treatment at pH 3 to 11. Both viruses were inactivated at pH 12, as was the human virus at pH 2, although this pH resulted in only partial inactivation of SA11. The human virus also appeared to be more sensitive than SA11 to the action of ether and chloroform. The infectivity of both viruses was lost after UV irradiation for 15 min and after treatment with 8% formaldehyde for 5 min, 70% (vol/vol) ethanol for 30 min, and 2% lysol, 2% phenol, and 1% H/sub 2/O/sub 2/ for 1 h each.

• Research Organization: Fairfield Hospital for Communicable Diseases, Victoria, Australia
• OSTI ID: 6498130
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 4; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALCOHOLS
AROMATICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CHLORINATED ALIPHATIC HYDROCARBONS
CHLOROFORM
DISINFECTANTS
ELECTROMAGNETIC RADIATION
ETHANOL
FLUORESCENCE
GERMICIDES
HALOGENATED ALIPHATIC HYDROCARBONS
HYDROXY COMPOUNDS
INFECTIVITY
LUMINESCENCE
MICROORGANISMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PARASITES
PHENOL
PHENOLS
RADIATION EFFECTS
RADIATIONS
TOXICITY
ULTRAVIOLET RADIATION
VIRUSES