Saturated fatty acid diet prevents radiation-associated decline in intestinal uptake

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Am. J. Physiol.; (United States)

OSTI ID:6496826

Thomson, A B; Keelan, M; Lam, T; Cheeseman, C I; Walker, K; Clandinin, M T

Adult female Sprague-Dawley rats were fed isocaloric semipurified diets containing a high content of either polyunsaturated (P) or saturated (S) fatty acids; these diets were nutritionally adequate, providing for all known essential nutrient requirements. On day 3 after beginning S or P, one group of animals was exposed to a single 6-Gy dose of abdominal radiation, and the other half was sham irradiated. S or P diets were continued for a further 14 days. Brush-border membrane purification and sucrase-specific activities were unaffected by diet or by abdominal irradiation. In rats fed P, irradiation was associated with an increase in jejunal brush-border membrane total phospholipid and the ratio of phospholipid to cholesterol; these changes were not observed in animals fed S. In irradiated rats, ileal brush-border membrane phospholipid per cholesterol was high in animals fed S compared with P. In irradiated animals fed P, there was reduced jejunal and ileal uptake of several medium- and long-chain saturated and unsaturated fatty acids and cholesterol, and the ileal uptake of higher concentrations of glucose was reduced in irradiated animals fed P. In contrast, lipid uptake was similar in control and irradiated animals fed S except for cholesterol uptake, which was reduced. Ileal uptake of higher concentrations of glucose was increased in irradiated animals fed S. Quantitative autoradiography failed to demonstrate any change in the distribution of leucine or lysine transport sites along the villus 1 or 2 wk after abdominal irradiation or in response to feeding S or P. Also, these differences in transport achieved by feeding S to radiated animals were not explained by variations in the animals' food consumption or intestinal mucosal surface area.

• Research Organization: Univ. of Alberta, Edmonton (Canada)
• OSTI ID: 6496826
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States) Vol. 256; ISSN AJPHA
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABSORPTION
ALDEHYDES
AMINO ACIDS
ANIMALS
AUTORADIOGRAPHY
CARBOHYDRATES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHOLESTEROL
DIET
ENZYMES
ESTERS
GLUCOSE
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
HYDROXY COMPOUNDS
INTESTINAL ABSORPTION
LIPIDS
MAMMALS
MEMBRANE TRANSPORT
MEMBRANES
MONOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
RADIOSENSITIVITY EFFECTS
RATS
RODENTS
SACCHARIDES
STEROIDS
STEROLS
UPTAKE
VERTEBRATES