Absorption of biliary cobalamin in baboons following total gastrectomy

Journal Article · Mon Nov 01 04:00:00 EST 1982 · J. Lab. Clin. Med.; (United States)

OSTI ID:6493044

Green, R; Jacobsen, D W; Van Tonder, S V; Kew, M C; Metz, J

Absorption of radiolabeled cobalamin in baboons was assessed by whole body counting. Retention of biliary cobalamin and an aqueous solution of cyanocobalamin was measured in normal baboons and in baboons after total gastrectomy by using /sup 57/Co-labeled biliary cobalamin and /sup 58/C0-cyanocobalamin, with and without baboon gastric juice containing intrinsic factor. Radiolabeled biliary cobalamin was obtained by intravenous injection of /sup 57/Co-cyanocobalamin in baboons and collection of bile through a cannula placed in the common bile duct. Cobalamin absorption was not completely abolished by gastrectomy and biliary cobalamin was better retained than cyanocobalamin; intrinsic factor enhanced absorption of both forms. After gastrectomy there was steady depletion of liver and serum cobalamin levels, which ceased after a new equilibrium was reached between a progressively diminishing cobalamin loss and the impaired but significant residual level of absorption. These studies in the nonhuman primate provide further information concerning the enterohepatic circulation of cobalamin and suggest that the form of cobalamin in bile may be more readily absorbed than is cyanocobalamin or that bile itself may have an enhancing effect on cobalamin absorption. The data also suggest that physiologically significant amounts of cobalamin may be absorbed in the absence of a gastric source of intrinsic factor.

• Research Organization: Department of Clinical Research, Scripps Clinic and Research Foundation, La Jolla, CA
• OSTI ID: 6493044
• Journal Information: J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 100:5; ISSN JLCMA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
ANIMALS
APES
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BILE
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBOHYDRATES
CHEMISTRY
COBALT 57
COBALT 58
COBALT ISOTOPES
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GASTRECTOMY
HEMATINICS
HEMATOLOGIC AGENTS
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
INTESTINAL ABSORPTION
INTRINSIC FACTOR
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MEDICINE
MUCOPROTEINS
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHYSIOLOGY
POLYSACCHARIDES
PRIMATES
PROTEINS
RADIOISOTOPES
SACCHARIDES
SURGERY
TRACER TECHNIQUES
UPTAKE
VERTEBRATES