Dietary and biliary cholesterol absorption in rats. Effect of dietary cholesterol level and cholesterol saturation of bile

Thesis/Dissertation · Tue Jan 01 04:00:00 EST 1985

OSTI ID:6067414

Wilson, M D

The principal objective of this research was to determine if cholesterol introduced into the duodenum of rats in a micellar form as occurs with bile, is absorbed more efficiently than cholesterol presented in a nonmicellar form, as occurs with dietary cholesterol. Cholesterol absorption was measured during the constant intraduodenal infusion of liquid diets ((/sup 14/C) cholesterol) and artificial biles ((/sup 3/H) cholesterol) in thoracic lymph duct cannulated rats. Percentage absorption was calculated by dividing the rate of appearance of radiolabeled cholesterol in lymph by its rate of infusion when lymph cholesterol specific activity was constant. Results provide strong evidence that under certain conditions biliary cholesterol is more efficiently absorbed than is dietary cholesterol, and that this differential must be considered when evaluating the influence of diet or drug therapy on cholesterol absorption.

• Research Organization: Bowman Gray School of Medicine, Winston-Salem, NC (USA)
• OSTI ID: 6067414
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
ANIMALS
BILE
BIOLOGICAL MATERIALS
BODY FLUIDS
CHOLESTEROL
DATA
DIET
EXPERIMENTAL DATA
HYDROXY COMPOUNDS
INFORMATION
INTESTINAL ABSORPTION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
NUMERICAL DATA
ORGANIC COMPOUNDS
RATS
RODENTS
STEROIDS
STEROLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES