Comparison of maximum production rates and optimum operating/design parameters in overloaded elution and displacement chromatography

Journal Article · Tue Jan 05 04:00:00 EST 1993 · Biotechnology and Bioengineering; (United States)

DOI:https://doi.org/10.1002/bit.260410118· OSTI ID:6487360

Felinger, A ^[1]; Guichon, G ^[2]

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1. Univ. of Tennessee, Knoxville (United States)
2. Oak Ridge National Lab., TN (United States)

The result of a study of the optimization of the experimental conditions for maximum production rate in overloaded elution and displacement chromatography are discussed. This study is based on the use of the equilibrium-dispersive model of chromatography and the competitive Langmuir isotherms to calculate individual band profiles in the elution and displacement modes, and of a simplex algorithm to optimize the production rate. The operating parameters (sample size, mobile phase velocity, and the displacer concentration in the displacement mode) and the column design parameters (column length and average particle diameter) are optimized simultaneously. Binary mixtures having relative concentrations 3:1 and 1:3, and separation factors of 1.2 to 1.8 are investigated. One of the major results is that, in both modes of chromatography, the maximum production rate is achieved at very low values of the retention factors, k[prime], much lower than those used in current practice. In all cases, unless k[prime] exceeds greatly that optimum value, the production rate is higher in overloaded elution than in displacement chromatography. This is particularly true for the extraction of a minor component, which is eluted second.

• OSTI ID: 6487360
• Journal Information: Biotechnology and Bioengineering; (United States), Journal Name: Biotechnology and Bioengineering; (United States) Vol. 41:1; ISSN BIBIAU; ISSN 0006-3592
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
ALGORITHMS
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
DESIGN
DISPERSIONS
EVALUATION
ISOTHERMS
MATHEMATICAL LOGIC
MATHEMATICAL MODELS
MIXTURES
OPTIMIZATION
SEPARATION PROCESSES