Reversible accumulation of double- and single-stranded DNA breaks in DNA in growth-arrested cells
Journal Article
·
· Bull. Exp. Biol. Med. (Engl. Transl.); (United States)
OSTI ID:6478288
The authors study the possibility of the formation and repair of double-stranded breaks in DNA during a change in the proliferative status of cells. Jungarian hamster fibroblasts, transformed by SV-40 virus, were cultured in Carrel's flasks in a nutrient mixture containing Eagle's medium, lactalbumin hydrolysate, and bovine serum in the ratio of 4.5:4.5:1. DNA was labelled by the addition of 0.4 MBq/ml of tritium-thymidine to the incubation medium for 2-7 days. To arrest cell growth, the monolayer formed seven days after seeding was placed in medium with the serum concentration lowered to 1%, and incubated for 7-9 days. The cells were restimulated for division with fresh complete medium. The mitotic index was 20% in the exponentially growing cultures while in the resting cultures it was 1-2% and rose to 16% 2 days after stimulation. Double-stranded DNA breaks were determined by neutral elution of DNA; single-stranded breaks were measured by the alkaline DNA uncoiling method with fixation of hydroxyapatite. The formation of double-stranded DNA breaks and their repair, in response to a change in the proliferative status of the cell, were discovered for the first time.
- Research Organization:
- Latvian Scientific-Research Institute of Experimental and Clinical Medicine, Riga, USSR
- OSTI ID:
- 6478288
- Journal Information:
- Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Journal Name: Bull. Exp. Biol. Med. (Engl. Transl.); (United States) Vol. 102:8; ISSN BEXBA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550301 -- Cytology-- Tracer Techniques
550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD SERUM
CELL CULTURES
CELL DIVISION
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DNA
FIBROBLASTS
HAMSTERS
HETEROCYCLIC COMPOUNDS
INHIBITION
LABELLED COMPOUNDS
LABELLING
MAMMALS
MITOSIS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRIMIDINES
RADIOASSAY
RECOVERY
REPAIR
RIBOSIDES
RODENTS
SOMATIC CELLS
STIMULATION
STRAND BREAKS
THYMIDINE
TRITIUM COMPOUNDS
VERTEBRATES
550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BLOOD SERUM
CELL CULTURES
CELL DIVISION
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DNA
FIBROBLASTS
HAMSTERS
HETEROCYCLIC COMPOUNDS
INHIBITION
LABELLED COMPOUNDS
LABELLING
MAMMALS
MITOSIS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PYRIMIDINES
RADIOASSAY
RECOVERY
REPAIR
RIBOSIDES
RODENTS
SOMATIC CELLS
STIMULATION
STRAND BREAKS
THYMIDINE
TRITIUM COMPOUNDS
VERTEBRATES