Effect of differentiation on the repair of DNA single strand breaks in neuroblastoma cells

Journal Article · Mon Apr 07 04:00:00 EDT 1975 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/S0006-291X(75)80444-X· OSTI ID:7187556

Byfield, J E; Lee, Y C; Klisak, I; Finklestein, J Z

The capacity of murine C-1300 neuroblastoma cells to repair x-ray induced DNA single strand breaks was compared under two distinct culture conditions. Growing undifferentiated cells maintained in normal medium containing 10 percent fetal calf serum showed substantial break rejoining during a 30 minute repair period. Non-growing, differentiated cells in serum-free medium did not appear to be capable of repairing such breaks. Whether this phenomenon is attributable to a loss of repair enzymes or to an increased lability of the differentiated cells related to adverse culture conditions is uncertain. The results suggest that nuclear integrity is not absolutely vital to morphogenetic differentiation of C-1300 neuroblasts and also imply that deterioration of tissue culture growth conditions may affect DNA repair mechanisms in a fashion independent of other complex biosynthetic events.

• Research Organization: Univ. of California, Los Angeles
• OSTI ID: 7187556
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 63:3; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHROMOSOMAL ABERRATIONS
CHROMOSOME BREAKAGE
DNA
IRRADIATION
MAMMALS
MICE
MUTATIONS
NERVE CELLS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
TISSUE CULTURES
VERTEBRATES