Radiobiology of ultrasoft X rays. I. Cultured hamster cells (V79)

Journal Article · Mon Jun 01 04:00:00 EDT 1987 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3577007· OSTI ID:6471964

Raju, M R; Carpenter, S G; Chmielewski, J J; Schillaci, M E; Wilder, M E; Freyer, J P; Johnson, N F; Schor, P L; Sebring, R J; Goodhead, D T

Ultrasoft X rays (approximately less than keV) provide a useful probe for the study of the physical parameters associated with the induction of biological lesions because the spatial scale of their energy depositions is of nanometer dimensions, comparable to that of critical structures within the cell. We report on cell-killing experiments using cultured hamster cells (V79) exposed to carbon K (0.28 keV), aluminum K (1.5 keV), copper K (8.0 keV), and 250 kVp X rays, under oxic and hypoxic conditions, and as a function of cell-cycle phase. Our principal results are: RBE increases with decreasing X-ray energy; OER decreases with decreasing X-ray energy; and cell-cycle response is similar for all X-ray energies. Our RBE results confirm earlier observations using ultrasoft X rays on mammalian cells. The shapes of fitted curves through the data for each energy are statistically indistinguishable from one another, implying that the enhanced effectiveness is purely dose modifying. The results reported herein generally support the view that single-track effects of radiation are predominantly due to very local energy depositions on the nanometer scale, which are principally responsible for observed radiobiological effects.

• Research Organization: Los Alamos National Lab., NM
• OSTI ID: 6471964
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALUMINIUM
ANIMAL CELLS
ANIMALS
ANOXIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGY
BODY
CARBON
CELL CYCLE
CELL KILLING
COPPER
ELECTROMAGNETIC RADIATION
ELEMENTS
HAMSTERS
IONIZING RADIATIONS
LUNGS
MAMMALS
METALS
NONMETALS
ORGANS
OXYGEN
RADIATION EFFECTS
RADIATIONS
RADIOBIOLOGY
RBE
RESPIRATORY SYSTEM
RODENTS
TRANSITION ELEMENTS
VERTEBRATES
X RADIATION