Measurement of DNA damage and cell killing in Chinese hamster V79 cells irradiated with aluminum characteristic ultrasoft X rays

Journal Article · Wed Mar 01 04:00:00 EST 1989 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3577354· OSTI ID:6253483

Prise, K M; Folkard, M; Davies, S; Michael, B D

Chinese hamster V79 cells were irradiated with 1.487 keV aluminum characteristic X rays produced using a cold-cathode discharge tube. Under aerobic conditions a relative biological effectiveness (RBE) of 2.18 for cell killing in comparison to 250-kVp X rays was measured using cells grown in suspension and irradiated on membrane filters. DNA damage in the form of single-strand (ssb) and double-strand breaks (dsb) was measured using the filter elution technique. The aerobic RBEs are 1.64 for dsb induction and 0.49 for ssb induction, consistent with the view that dsb are more closely related to cell kill than ssb. A reduced oxygen enhancement ratio (OER) for cell killing was measured for Al-K X rays, but the OER for dsb induction was similar to that measured for 250-kVp X rays. A curvilinear relationship between dsb induction and dose is observed, similar to that seen for 250-kVp X rays. This agrees with the concept that ultrasoft X rays produce critical lesions similar to hard X rays but with a greater efficiency per unit dose.

• Research Organization: Mount Vernon Hospital, Northwood, Middlesex (England)
• OSTI ID: 6253483
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 117:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROBIC CONDITIONS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CELL KILLING
DNA
ELECTROMAGNETIC RADIATION
ELEMENTS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
HAMSTERS
IONIZING RADIATIONS
LUNGS
MAMMALS
NONMETALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXYGEN
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RBE
RESPIRATORY SYSTEM
RODENTS
STRAND BREAKS
VERTEBRATES
X RADIATION