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Stimulation of aortic smooth muscle cell mitogenesis by serotonin

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ; ;
Bovine aortic smooth muscle cells in vitro responded to 1 nM to 10 ..mu..M serotonin with increased incorporation of (/sup 3/H)thymidine into DNA. The mitogenic effect of serotonin was half-maximal at 80 nM and maximal above 1 ..mu..M. At a concentration of 1 ..mu..M, serotonin stimulated smooth muscle cell mitogenesis to the same extent as human platelet-derived growth factor (PDGF) at 12 ng/ml. Tryptamine was approx. = 1/10th as potent as serotonin as a mitogen for smooth muscle cells. Other indoles that are structurally related to serotonin (D- and L-tryptophan, 5-hydroxy-L-tryptophan, N-acetyl-5-hydroxytryptamine, melatonin, 5-hydroxyindoleacetic acid, and 5-hydroxytryptophol) and quipazine were inactive. The stimulatory effect of serotonin on smooth muscle cell DNA synthesis required prolonged (20-24 hr) exposure to the agonist and was attenuated in the presence of serotonin D receptor antagonists. When smooth muscle cells were incubated with submaximal concentrations of serotonin and PDGF, synergistic rather than additive mitogenic responses were observed. These data indicate that serotonin has a significant mitogenic effect on smooth muscle cells in vitro, which appears to be mediated by specific plasma membrane receptors.
Research Organization:
Sandoz Research Institute, East Hanover, NJ
OSTI ID:
6470123
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 83:3; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
ANIMALS
AORTA
AROMATICS
ARTERIES
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZINES
AZOLES
BIOLOGICAL EFFECTS
BLOOD VESSELS
BODY
CARDIOVASCULAR SYSTEM
CATTLE
CELL DIVISION
DNA
DOMESTIC ANIMALS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
INDOLES
LABELLED COMPOUNDS
MAMMALS
MITOGENS
MITOSIS
MUSCLES
NEUROREGULATORS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PYRIMIDINES
PYRROLES
RADIOPROTECTIVE SUBSTANCES
RIBOSIDES
RUMINANTS
SEROTONIN
SYMPATHOMIMETICS
THYMIDINE
TRITIUM COMPOUNDS
TRYPTAMINES
VERTEBRATES