Identification and cytotoxic reactivity of inflammatory cells recovered from progressing or regressing syngeneic uv-induced murine tumors
Journal Article
·
· J. Immunol.; (United States)
OSTI ID:6469874
Most tumors induced in C3H mice by ultraviolet (uv) light are immunologically rejected by normal syngeneic recipients, but will grow progressively in ummunosuppressed mice and in mice treated with uv light. In this study we compared the composition and cytotoxic activity of the inflammatory cell infiltrate from tumors transplanted into syngeneic uv-irradiated or unirradiated mice. Tumor fragments were implanted in either normal (regressor) or uv-treated (progressor) mice, and removed on various days after implantation and mechanically dissociated. The cells were examined by immunofluorescence for theta and immunoglobulin markers, stained for morphologic examination, and tested for cytotoxicity against the tumor. No significant differences were noted in numbers of macrophages, granulocytes, or B cells recovered from progressing or regressing tumors on day 6, the time of greatest activity. However, the numbers of T cells recovered from tumor fragments implanted in normal mice was approximately 3-fold that recovered from tumor fragments implanted in uv-treated mice. Lymphocytes recovered from regressing tumor fragments were specifically cytotoxic for that tumor in a microcytotoxicity test; those from progressing tumor fragments were not cytotoxic.
- Research Organization:
- National Cancer Inst., Frederick, MD
- OSTI ID:
- 6469874
- Journal Information:
- J. Immunol.; (United States), Journal Name: J. Immunol.; (United States) Vol. 121:5; ISSN JOIMA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
IMMUNE REACTIONS
LEUKOCYTES
LYMPHOCYTES
MACROPHAGES
MAMMALS
MICE
MORPHOLOGICAL CHANGES
NEOPLASMS
PHAGOCYTES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RODENTS
SOMATIC CELLS
THYMOCYTES
TOXICITY
TRANSPLANTS
TUMOR CELLS
ULTRAVIOLET RADIATION
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
IMMUNE REACTIONS
LEUKOCYTES
LYMPHOCYTES
MACROPHAGES
MAMMALS
MICE
MORPHOLOGICAL CHANGES
NEOPLASMS
PHAGOCYTES
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RODENTS
SOMATIC CELLS
THYMOCYTES
TOXICITY
TRANSPLANTS
TUMOR CELLS
ULTRAVIOLET RADIATION
VERTEBRATES