Biochemical mechanisms underlying the development of radioresistance by cultured peritoneal exudate macrophages

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3577278· OSTI ID:6466985

Lin, H S; Hsu, S

We investigated changes in radiosensitivity of peritoneal exudate macrophage colony-forming cells (PE-CFC) when exudative peritoneal macrophages were cultured in vitro. The change in the shape of the dose-response curve of PE-CFC to ionizing irradiation was partly dependent on the concentration of oxygen in the gas phase of the incubators. When cells were incubated in an environment containing 20% oxygen, the value of both Dq and D0 for PE-CFC increased. The dose-response curve of PE-CFC cultured for 3 days resembled that of alveolar macrophage colony-forming cells (AL-CFC). The changes in radiosensitivity were accompanied by an increase in the level of three antioxidant enzymes: superoxide dismutase, catalase, and glutathione peroxidase. However, when they were cultured in a 6% oxygen environment, only the value of Dq increased. When alveolar macrophages were incubated in vitro, no significant change in the shape of the dose-response curve of AL-CFC was noted whether they were cultured in gas phase containing either 20 or 6% oxygen. It is concluded that the radiosensitivity of PE-CFC changes when they are cultured in vitro. The increase in D0 appears to be related to the intracellular level of antioxidant enzymes.

• Research Organization: Washington Univ. School of Medicine, St. Louis, MO (USA)
• OSTI ID: 6466985
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 117:1; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
CATALASE
COLONY FORMATION
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
DRUGS
ELEMENTS
ENZYMES
GLUTATHIONE
IN VITRO
IONIZING RADIATIONS
MACROPHAGES
MAMMALS
MICE
NONMETALS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
OXYGEN
PEPTIDES
PEROXIDASES
PHAGOCYTES
POLYPEPTIDES
PROTEINS
RADIATIONS
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIVITY
RODENTS
SOMATIC CELLS
SUPEROXIDE DISMUTASE
VERTEBRATES