Cell killing and mutation to 6-thioguanine resistance after exposure to tritiated amino acids and tritiated thymidine in cultured mammalian cells (L5178Y)
Cell killing and mutation to 6-thioguanine resistance were studied in growing mouse leukemia cells in culture after exposure to tritiated amino acids and tritiated thymidine. These effects varied widely among the tritiated compounds tested, being greatest for tritiated thymidine followed by tritiated arginine and tritiated lysine, in that order, for a given concentration of /sup 3/H expressed in kBq/ml of /sup 3/H in the medium. The differences between each tritiated amino acid disappeared almost totally when the effects were compared on the basis of the absorbed dose to the cells. The effects of tritiated thymidine, however, remained more than twofold greater compared to other tritiated compounds. These results indicate the importance of determining the absorbed dose for assessment of the radiotoxicity of tritiated organic compounds. For an exceptional case (tritiated thymidine), contribution of a mechanism(s) other than beta irradiation should also be taken into account.
- Research Organization:
- National Institute of Radiological Sciences, Chiba-shi, Japan
- OSTI ID:
- 6465853
- Journal Information:
- Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 3; ISSN RAREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
Kinetics
& Toxicology-- Animals
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Microorganisms
& Cells
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AROMATICS
AZAARENES
AZINES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBOXYLIC ACIDS
CELL CULTURES
CELL KILLING
DISEASES
GUANINE
HEMIC DISEASES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INJURIES
LABELLED COMPOUNDS
LEUKEMIA
MAMMALS
MICE
MUTATIONS
NEOPLASMS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PURINES
PYRIMIDINES
RADIATION EFFECTS
RADIATION INJURIES
RIBOSIDES
RODENTS
SURVIVAL TIME
THYMIDINE
TOXICITY
TRITIUM COMPOUNDS
TUMOR CELLS
VERTEBRATES