Unfractionated human thymocytes have a lower proliferative capacity than CD3/sup -/4/sup -/8/sup -/ ones but have a similar capacity for expression of interleukin 2 receptors and production of interleukin 2
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
CD3/sup -/4/sup -/8/sup -/ and unfractionated thymocytes were compared for their capacity to proliferate, to express interleukin 2 (IL-2) receptor, and to secrete IL-2. Phorbol ester and Ca/sup 2 +/ ionophore were used as mitogens. CD3/sup -/4/sup -/8/sup -/ thymocytes responded vigorously when stimulated with phorbol ester in the presence of IL-2 or in combination with Ca/sup 2 +/ ionophore. In contrast, unfractionated thymocytes responded weakly when stimulated with either of these mitogens. Surprisingly, however, the stimulation of these populations with either phorbol ester plus IL-2 or phorbol ester plus ionophore induced a high and similar level of IL-2 receptor expression in both thymocyte populations. A similar level of IL-2 secretion in both populations was also obtained when they were stimulated with a combination of phorbol ester plus ionophere. These results suggest that during the maturation process, the majority of thymocytes lose their capacity to be activated by some mitogens, although they maintain their capacity to secrete IL-2 and to express the IL-2 receptor.
- Research Organization:
- Hospital Clinic i Provincial, Barcelona (Spain)
- OSTI ID:
- 6460359
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:23; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGE GROUPS
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
CALCIUM COMPOUNDS
CARCINOGENS
CATIONS
CELL CULTURES
CELL PROLIFERATION
CHARGED PARTICLES
CHEMISTRY
CHILDREN
ESTERS
GROWTH FACTORS
IONS
LYMPHOKINES
MEMBRANE PROTEINS
MITOGENS
ORGANIC COMPOUNDS
PATIENTS
PHORBOL ESTERS
PROTEINS
RECEPTORS
SOMATIC CELLS
THYMOCYTES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGE GROUPS
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
BIOCHEMISTRY
BIOLOGICAL EFFECTS
CALCIUM COMPOUNDS
CARCINOGENS
CATIONS
CELL CULTURES
CELL PROLIFERATION
CHARGED PARTICLES
CHEMISTRY
CHILDREN
ESTERS
GROWTH FACTORS
IONS
LYMPHOKINES
MEMBRANE PROTEINS
MITOGENS
ORGANIC COMPOUNDS
PATIENTS
PHORBOL ESTERS
PROTEINS
RECEPTORS
SOMATIC CELLS
THYMOCYTES