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Concanavalin A induced phosphatidyl inositol turnover in mouse thymocytes is not coupled to activation of protein kinase C or mitogenic response to interleukin 2

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5148728
;
The authors have examined the connection between phosphoinositide metabolism and lectin-activation of T-cells by measuring in vivo incorporation of /sup 32/PO/sub 4/ into phospholipids. Mouse thymocytes treated with Concanavalin A (Con A) do not proliferate nor develop mitogenic responsiveness to Interleukin 2 (IL-2). Co-treatment with Con A and either activated splenocyte conditioned medium (CM) or the Protein Kinase C (PK-C) activator, Phorbol Myristic Acetate (PMA), induces IL-2 responsiveness with a parallel increase in expression of IL-2 surface responsiveness with a parallel increase in expression of IL-2 surface receptors. Con A alone stimulates phosphoinositol (PI) turnover by 2.5-3.7 fold but has no effect on incorporation of /sup 32/PO/sub 4/ into other phospholipids, while PI turnover is unaffected by CM with or without Con A. Diacyl Glycerol (DG) arising from the breakdown of phosphoinositides, is postulated to activate PK-C (as is also PMA). These data suggest that PI breakdown, stimulated by Con A, does not produce sufficient DG to activate PK-C and/or PK-C activation by itself is not sufficient to activate IL-2 responsiveness and the generation of IL-2 receptors. The growth factor(s) in CM also do not appear to activate PK-C but do co-activate IL-2 responsiveness. The authors have observed that CM or PMA but not Con A treatment of thymocytes specifically stimulates the phosphorylation of a 55Kd polypeptide suggesting that other kinases with similar specificities to PK-C are activated by CM and PMA.
Research Organization:
Duke Univ. Medical Center, Durham, NC
OSTI ID:
5148728
Report Number(s):
CONF-8606151-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AGGLUTININS
ALCOHOLS
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARCINOGENS
CELL PROLIFERATION
CONCANAVALIN
CONNECTIVE TISSUE CELLS
ENZYME ACTIVITY
ENZYMES
ESTERS
GLYCEROL
GROWTH FACTORS
HEMAGGLUTININS
HYDROXY COMPOUNDS
LECTINS
LEUKOCYTES
LIPIDS
LYMPHOCYTES
LYMPHOKINES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
METABOLISM
MICE
MITOGENS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXYGEN COMPOUNDS
PHORBOL ESTERS
PHOSPHATES
PHOSPHOLIPIDS
PHOSPHORUS COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
PROTEINS
RECEPTORS
RODENTS
SOMATIC CELLS
TRANSFERASES
VERTEBRATES