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Phenylephrine stimulated breakdown of phosphoinositides in brown adipocytes is attenuated by adenosine

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:5321927
Selective activation of alpha adrenergic receptors on brown adipocytes brings about increased mitochondrial respiration. This response is associated with a rapid breakdown of phosphoinositides in the plasma membrane. The authors have shown that respiration increased by alpha receptor activation can be inhibited by adenosine but the mechanisms underlying this effect are unknown. The present study probes the possibility that adenosine inhibition of alpha receptor stimulated respiration is secondary to an inhibition of stimulated breakdown of inositol phospholipids. Phospholipids were labeled with (/sup 32/P) by incubation with (/sup 32/P)-Pi for up to four hours. Phenylephrine and other ligands were then added and the radioactivity present in individual lipids determined following their resolution by thin layer chromatography. Addition of 2-chloroadenosine or phenylisopropyl adenosine, but not 2',5'-dideoxyadenosine, inhibited phenylephrine promoted breakdown of phosphoinositides. The dose response relation for this effect was similar to that for attenuation of stimulated respiration. This finding demonstrates adenosine inhibition of a phospholipase in brown fat cells and suggests the possibility that breakdown of inositol phospholipids is a critical control site for stimulation and attenuation of respiration.
Research Organization:
UMDNJ-Rutgers Medical School, Piscataway, NJ
OSTI ID:
5321927
Report Number(s):
CONF-8604222-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:3
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
ADIPOSE TISSUE
ANIMAL TISSUES
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
CHROMATOGRAPHY
CONNECTIVE TISSUE
DAYS LIVING RADIOISOTOPES
DRUGS
ESTERS
INHIBITION
INOSITOL
INOSITOLS
ISOTOPES
LABELLING
LIGANDS
LIGHT NUCLEI
LIPIDS
MEMBRANE PROTEINS
MEMBRANES
METABOLISM
MITOCHONDRIA
MONOSACCHARIDES
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RECEPTORS
RESPIRATION
RIBOSIDES
SACCHARIDES
SEPARATION PROCESSES
SYMPATHOMIMETICS
THIN-LAYER CHROMATOGRAPHY
TISSUES