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Title: Hybrid slab-microchannel gel electrophoresis system

Abstract

A hybrid slab-microchannel gel electrophoresis system is described. The hybrid system permits the fabrication of isolated microchannels for biomolecule separations without imposing the constraint of a totally sealed system. The hybrid system is reusable and ultimately much simpler and less costly to manufacture than a closed channel plate system. The hybrid system incorporates a microslab portion of the separation medium above the microchannels, thus at least substantially reducing the possibility of non-uniform field distribution and breakdown due to uncontrollable leakage. A microslab of the sieving matrix is built into the system by using plastic spacer materials and is used to uniformly couple the top plate with the bottom microchannel plate. 4 figs.

Inventors:
; ; ;
Publication Date:
Research Org.:
University of California
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
645954
Patent Number(s):
US 5,746,901/A/
Application Number:
PAN: 8-628,309
Assignee:
Univ. Of California, Oakland, CA (United States) PTO; SCA: 400105; PA: EDB-98:101504; SN: 98001993255
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 5 May 1998
Country of Publication:
United States
Language:
English
Subject:
40 CHEMISTRY; ELECTROPHORESIS; SEPARATION EQUIPMENT; HYBRID SYSTEMS; DESIGN; GELS

Citation Formats

Balch, J.W., Carrano, A.V., Davidson, J.C., and Koo, J.C. Hybrid slab-microchannel gel electrophoresis system. United States: N. p., 1998. Web.
Balch, J.W., Carrano, A.V., Davidson, J.C., & Koo, J.C. Hybrid slab-microchannel gel electrophoresis system. United States.
Balch, J.W., Carrano, A.V., Davidson, J.C., and Koo, J.C. Tue . "Hybrid slab-microchannel gel electrophoresis system". United States.
@article{osti_645954,
title = {Hybrid slab-microchannel gel electrophoresis system},
author = {Balch, J.W. and Carrano, A.V. and Davidson, J.C. and Koo, J.C.},
abstractNote = {A hybrid slab-microchannel gel electrophoresis system is described. The hybrid system permits the fabrication of isolated microchannels for biomolecule separations without imposing the constraint of a totally sealed system. The hybrid system is reusable and ultimately much simpler and less costly to manufacture than a closed channel plate system. The hybrid system incorporates a microslab portion of the separation medium above the microchannels, thus at least substantially reducing the possibility of non-uniform field distribution and breakdown due to uncontrollable leakage. A microslab of the sieving matrix is built into the system by using plastic spacer materials and is used to uniformly couple the top plate with the bottom microchannel plate. 4 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1998},
month = {5}
}