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Title: System for loading slab-gel holders for electrophoresis separation

Abstract

A slab-gel loading system includes a prismatic chamber for filling a plurality of slab-gel holders simultaneously. Each slab-gel holder comprises a pair of spaced apart plates defining an intermediate volume for gel containment. The holders are vertically positioned in the chamber with their major surfaces parallel to the chamber end walls. A liquid inlet is provided at the corner between the bottom and a side wall of the chamber for distributing a polymerizable monomer solution or a coagulable colloidal solution into each of the holders. The chamber is rotatably supported so that filling can begin with the corner having the liquid inlet directed downwardly such that the solution is gently funneled upwardly, without mixing, along the diverging side and bottom surfaces. As filling proceeds, the chamber is gradually rotated to position the bottom wall in a horizontal mode. The liquid filling means includes a plastic envelope with a septum dividing it into two compartments for intermixing two solutions of different density and thereby providing a liquid flow having a density gradient. The resulting gels have a density gradient between opposite edges for subsequent use in electrophoresis separations.

Inventors:
 [1];  [2]
  1. (Hinsdale, IL)
  2. (Willowbrook, IL)
Publication Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL
OSTI Identifier:
863428
Patent Number(s):
US 4169036
Assignee:
United States of America as represented by United States (Washington, DC) ANL
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
loading; slab-gel; holders; electrophoresis; separation; prismatic; chamber; filling; plurality; simultaneously; holder; comprises; pair; spaced; apart; plates; defining; intermediate; volume; containment; vertically; positioned; major; surfaces; parallel; walls; liquid; inlet; provided; corner; bottom; wall; distributing; polymerizable; monomer; solution; coagulable; colloidal; rotatably; supported; begin; directed; downwardly; gently; funneled; upwardly; mixing; diverging; proceeds; gradually; rotated; position; horizontal; mode; means; plastic; envelope; septum; dividing; compartments; intermixing; solutions; density; providing; flow; gradient; resulting; gels; opposite; edges; subsequent; separations; bottom wall; rotatably supported; major surfaces; liquid inlet; liquid flow; bottom surfaces; spaced apart; bottom surface; opposite edges; monomer solution; density gradient; major surface; resulting gels; slab-gel holders; colloidal solution; opposite edge; electrophoresis separation; liquid filling; electrophoresis separations; vertically position; slab-gel holder; /425/204/

Citation Formats

Anderson, Norman G., and Anderson, Norman L. System for loading slab-gel holders for electrophoresis separation. United States: N. p., 1979. Web.
Anderson, Norman G., & Anderson, Norman L. System for loading slab-gel holders for electrophoresis separation. United States.
Anderson, Norman G., and Anderson, Norman L. Mon . "System for loading slab-gel holders for electrophoresis separation". United States. https://www.osti.gov/servlets/purl/863428.
@article{osti_863428,
title = {System for loading slab-gel holders for electrophoresis separation},
author = {Anderson, Norman G. and Anderson, Norman L.},
abstractNote = {A slab-gel loading system includes a prismatic chamber for filling a plurality of slab-gel holders simultaneously. Each slab-gel holder comprises a pair of spaced apart plates defining an intermediate volume for gel containment. The holders are vertically positioned in the chamber with their major surfaces parallel to the chamber end walls. A liquid inlet is provided at the corner between the bottom and a side wall of the chamber for distributing a polymerizable monomer solution or a coagulable colloidal solution into each of the holders. The chamber is rotatably supported so that filling can begin with the corner having the liquid inlet directed downwardly such that the solution is gently funneled upwardly, without mixing, along the diverging side and bottom surfaces. As filling proceeds, the chamber is gradually rotated to position the bottom wall in a horizontal mode. The liquid filling means includes a plastic envelope with a septum dividing it into two compartments for intermixing two solutions of different density and thereby providing a liquid flow having a density gradient. The resulting gels have a density gradient between opposite edges for subsequent use in electrophoresis separations.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1979},
month = {1}
}

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