System for loading slab-gel holders for electrophoresis separation
- Hinsdale, IL
- Willowbrook, IL
A slab-gel loading system includes a prismatic chamber for filling a plurality of slab-gel holders simultaneously. Each slab-gel holder comprises a pair of spaced apart plates defining an intermediate volume for gel containment. The holders are vertically positioned in the chamber with their major surfaces parallel to the chamber end walls. A liquid inlet is provided at the corner between the bottom and a side wall of the chamber for distributing a polymerizable monomer solution or a coagulable colloidal solution into each of the holders. The chamber is rotatably supported so that filling can begin with the corner having the liquid inlet directed downwardly such that the solution is gently funneled upwardly, without mixing, along the diverging side and bottom surfaces. As filling proceeds, the chamber is gradually rotated to position the bottom wall in a horizontal mode. The liquid filling means includes a plastic envelope with a septum dividing it into two compartments for intermixing two solutions of different density and thereby providing a liquid flow having a density gradient. The resulting gels have a density gradient between opposite edges for subsequent use in electrophoresis separations.
- Research Organization:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Assignee:
- United States of America as represented by United States (Washington, DC)
- Patent Number(s):
- US 4169036
- OSTI ID:
- 863428
- Country of Publication:
- United States
- Language:
- English
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System for loading slab-gel holders for electrophoresis separation
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Related Subjects
slab-gel
holders
electrophoresis
separation
prismatic
chamber
filling
plurality
simultaneously
holder
comprises
pair
spaced
apart
plates
defining
intermediate
volume
containment
vertically
positioned
major
surfaces
parallel
walls
liquid
inlet
provided
corner
bottom
wall
distributing
polymerizable
monomer
solution
coagulable
colloidal
rotatably
supported
begin
directed
downwardly
gently
funneled
upwardly
mixing
diverging
proceeds
gradually
rotated
position
horizontal
mode
means
plastic
envelope
septum
dividing
compartments
intermixing
solutions
density
providing
flow
gradient
resulting
gels
opposite
edges
subsequent
separations
bottom wall
rotatably supported
major surfaces
liquid inlet
liquid flow
bottom surfaces
spaced apart
bottom surface
opposite edges
monomer solution
density gradient
major surface
resulting gels
slab-gel holders
colloidal solution
opposite edge
electrophoresis separation
liquid filling
electrophoresis separations
vertically position
slab-gel holder
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