skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Hybrid slab-microchannel gel electrophoresis system

Abstract

A hybrid slab-microchannel gel electrophoresis system. The hybrid system permits the fabrication of isolated microchannels for biomolecule separations without imposing the constraint of a totally sealed system. The hybrid system is reusable and ultimately much simpler and less costly to manufacture than a closed channel plate system. The hybrid system incorporates a microslab portion of the separation medium above the microchannels, thus at least substantially reducing the possibility of non-uniform field distribution and breakdown due to uncontrollable leakage. A microslab of the sieving matrix is built into the system by using plastic spacer materials and is used to uniformly couple the top plate with the bottom microchannel plate.

Inventors:
 [1];  [1];  [1];  [2]
  1. (Livermore, CA)
  2. (San Ramon, CA)
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
OSTI Identifier:
871521
Patent Number(s):
US 5746901
Assignee:
Regents Of University Of California (Oakland, CA) LLNL
DOE Contract Number:
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
hybrid; slab-microchannel; electrophoresis; permits; fabrication; isolated; microchannels; biomolecule; separations; imposing; constraint; totally; sealed; reusable; ultimately; simpler; costly; manufacture; closed; channel; plate; incorporates; microslab; portion; separation; medium; substantially; reducing; possibility; non-uniform; field; distribution; breakdown; due; uncontrollable; leakage; sieving; matrix; built; plastic; spacer; materials; uniformly; couple; top; bottom; microchannel; channel plate; microchannel plate; top plate; substantially reducing; spacer material; uniform field; hybrid slab-microchannel; field distribution; /204/

Citation Formats

Balch, Joseph W., Carrano, Anthony V., Davidson, James C., and Koo, Jackson C.. Hybrid slab-microchannel gel electrophoresis system. United States: N. p., 1998. Web.
Balch, Joseph W., Carrano, Anthony V., Davidson, James C., & Koo, Jackson C.. Hybrid slab-microchannel gel electrophoresis system. United States.
Balch, Joseph W., Carrano, Anthony V., Davidson, James C., and Koo, Jackson C.. Thu . "Hybrid slab-microchannel gel electrophoresis system". United States. doi:. https://www.osti.gov/servlets/purl/871521.
@article{osti_871521,
title = {Hybrid slab-microchannel gel electrophoresis system},
author = {Balch, Joseph W. and Carrano, Anthony V. and Davidson, James C. and Koo, Jackson C.},
abstractNote = {A hybrid slab-microchannel gel electrophoresis system. The hybrid system permits the fabrication of isolated microchannels for biomolecule separations without imposing the constraint of a totally sealed system. The hybrid system is reusable and ultimately much simpler and less costly to manufacture than a closed channel plate system. The hybrid system incorporates a microslab portion of the separation medium above the microchannels, thus at least substantially reducing the possibility of non-uniform field distribution and breakdown due to uncontrollable leakage. A microslab of the sieving matrix is built into the system by using plastic spacer materials and is used to uniformly couple the top plate with the bottom microchannel plate.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1998},
month = {Thu Jan 01 00:00:00 EST 1998}
}

Patent:

Save / Share: