Modulation of growth, differentiation, and mucous glycoprotein synthesis by retinyl acetate in cloned carcinoma cell lines. [Rats]

Journal Article · Mon Jun 01 00:00:00 EDT 1981 · J. Natl. Cancer Inst.; (United States)

OSTI ID:6450255

Marchok, A C; Clark, J N; Klein-Szanto, A

The ability of retinyl acetate to alter growth, differentiation, and synthesis of mucous glycoproteins in cell lines cloned from an adenocarcinoma (T-8) and a squamous cell carcinoma (1000 WT) was investigated with the use of F344 rats. Growth rate was inhibited approximately 25 and 50% in 6.6 x 10/sup -6/ and 3.3 x 10/sup -5/ M retinyl acetate, respectively, in both cell lines. Retinyl acetate induced the formation of numerous vacuoles and periodic acid-silver methenamine-positive granules in both T-8 and 1000 WT cells. After T-8 cells were cultured for 7 days in retinyl acetate, (/sup 3/H)glucosamine incorporation increased 133- to 147-fold and (/sup 14/C)serine incorporation increased twelvefold to twentyfold in the high-molecular-weight mucous glycoprotein fraction (peak A) from the cell cytosol. In 1000 WT cells, (/sup 3/H)glucosamine incorporation increased only 4.2- to 7.5-fold, and (/sup 14/C)serine incorporation increased only 2.6- to 4.6-fold under the same culture conditions. Thus T-8 cells showed a marked increase in the synthesis and secretion of mucins, whereas 1000 WT cells showed a comparatively small but significant increase.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6450255
• Journal Information: J. Natl. Cancer Inst.; (United States), Vol. 66:6
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
GLUCOPROTEINS
BIOSYNTHESIS
TUMOR CELLS
CELL DIFFERENTIATION
CELL PROLIFERATION
VITAMIN A
BIOLOGICAL EFFECTS
ACETATES
CARBON 14 COMPOUNDS
CARCINOMAS
CLONE CELLS
GLUCOSAMINE
RATS
TRACER TECHNIQUES
TRANSMISSION ELECTRON MICROSCOPY
TRITIUM COMPOUNDS
ULTRASTRUCTURAL CHANGES
AMINES
ANIMAL CELLS
ANIMALS
CARBOHYDRATES
CARBOXYLIC ACID SALTS
CELL CULTURES
DISEASES
ELECTRON MICROSCOPY
HEXOSAMINES
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MICROSCOPY
MONOSACCHARIDES
MORPHOLOGICAL CHANGES
NEOPLASMS
ORGANIC COMPOUNDS
PROTEINS
RODENTS
SACCHARIDES
SYNTHESIS
VERTEBRATES
VITAMINS
550301* - Cytology- Tracer Techniques
560301 - Chemicals Metabolism & Toxicology- Cells- (-1987)