Mucin secretion is stimulated by purinergic drugs: Studies with hamster tracheal surface epithelial (HTSE) cells in primary culture
Conference
·
· FASEB Journal (Federation of American Societies for Experimental Biology); (United States)
OSTI ID:5617275
- Boston Univ. School of Medicine, MA (United States)
Mucous glycoproteins or mucins are a major component of the mucus and are responsible for the viscoelastic property of the mucus. Confluent primary cultures of HTSE cells are highly enriched with secretory cells and secrete mucins, resembling airway goblet cells (Kim et al, JBC 260:4021, 1985). Using this cell culture system, the authors examined effects of purinergic drugs on mucin secretion. Confluent HTSE cells were metabolically labeled with 3H-glucosamine for 24 h and chased under various drug treatments. 3H-Mucins were quantified by Sepharose CL-4B gel-filtration column chromatography eluting with 50 mM sodium acetate/0.1% SDS. They found that 20 uM ATP stimulates mucin secretion by two fold while the same concentration of adenosine shows no effect. The order of potency was ATP>GTP=GDP{much gt}adenosine. Both ATP{sub {alpha}}S and GTP{sub {alpha}}S, non-hydrolyzable forms of ATP and GTP respectively, had almost identical potencies with their hydrolyzable counterparts indicating that the stimulatory effect of these nucleotides does not require the hydrolysis of high energy phosphate groups. They conclude that HTSE cells contain P2 receptors stimulation of which results in secretion of mucins. This may be an important mechanism by which airway goblet cell mucin secretion is regulated in vivo.
- OSTI ID:
- 5617275
- Report Number(s):
- CONF-9004153--
- Conference Information:
- Journal Name: FASEB Journal (Federation of American Societies for Experimental Biology); (United States) Journal Volume: 4:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AROMATICS
AZAARENES
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CELL CULTURES
CHEMICAL COMPOSITION
DRUGS
EPITHELIUM
GLYCOPROTEINS
HAMSTERS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PURINES
RESPIRATORY SYSTEM
RODENTS
STIMULATION
TISSUES
TRACER TECHNIQUES
TRACHEA
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AROMATICS
AZAARENES
BIOLOGICAL FUNCTIONS
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CELL CULTURES
CHEMICAL COMPOSITION
DRUGS
EPITHELIUM
GLYCOPROTEINS
HAMSTERS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROTEINS
PURINES
RESPIRATORY SYSTEM
RODENTS
STIMULATION
TISSUES
TRACER TECHNIQUES
TRACHEA
TRITIUM COMPOUNDS
VERTEBRATES