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Modulation of growth, differentiation, and mucous glycoprotein synthesis by retinyl acetate in cloned carcinoma cell lines. [Rats]

Journal Article · · J. Natl. Cancer Inst.; (United States)
OSTI ID:6450255
; ;
The ability of retinyl acetate to alter growth, differentiation, and synthesis of mucous glycoproteins in cell lines cloned from an adenocarcinoma (T-8) and a squamous cell carcinoma (1000 WT) was investigated with the use of F344 rats. Growth rate was inhibited approximately 25 and 50% in 6.6 x 10/sup -6/ and 3.3 x 10/sup -5/ M retinyl acetate, respectively, in both cell lines. Retinyl acetate induced the formation of numerous vacuoles and periodic acid-silver methenamine-positive granules in both T-8 and 1000 WT cells. After T-8 cells were cultured for 7 days in retinyl acetate, (/sup 3/H)glucosamine incorporation increased 133- to 147-fold and (/sup 14/C)serine incorporation increased twelvefold to twentyfold in the high-molecular-weight mucous glycoprotein fraction (peak A) from the cell cytosol. In 1000 WT cells, (/sup 3/H)glucosamine incorporation increased only 4.2- to 7.5-fold, and (/sup 14/C)serine incorporation increased only 2.6- to 4.6-fold under the same culture conditions. Thus T-8 cells showed a marked increase in the synthesis and secretion of mucins, whereas 1000 WT cells showed a comparatively small but significant increase.
Research Organization:
Oak Ridge National Lab., TN
DOE Contract Number:
W-7405-ENG-26
OSTI ID:
6450255
Journal Information:
J. Natl. Cancer Inst.; (United States), Journal Name: J. Natl. Cancer Inst.; (United States) Vol. 66:6; ISSN JNCIA
Country of Publication:
United States
Language:
English

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Related Subjects

550301* -- Cytology-- Tracer Techniques
560301 -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETATES
AMINES
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACID SALTS
CARCINOMAS
CELL CULTURES
CELL DIFFERENTIATION
CELL PROLIFERATION
CLONE CELLS
DISEASES
ELECTRON MICROSCOPY
GLUCOPROTEINS
GLUCOSAMINE
HEXOSAMINES
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MICROSCOPY
MONOSACCHARIDES
MORPHOLOGICAL CHANGES
NEOPLASMS
ORGANIC COMPOUNDS
PROTEINS
RATS
RODENTS
SACCHARIDES
SYNTHESIS
TRACER TECHNIQUES
TRANSMISSION ELECTRON MICROSCOPY
TRITIUM COMPOUNDS
TUMOR CELLS
ULTRASTRUCTURAL CHANGES
VERTEBRATES
VITAMIN A
VITAMINS