Isolation of basal and mucous cell populations from rabbit trachea
Journal Article
·
· Am. Rev. Respir. Dis.; (United States)
OSTI ID:5466999
The application of a unit gravity sedimentation procedure to monodispersed rabbit tracheal cells resulted in the isolation of enriched (2-fold to 2.5-fold) basal and mucous cell populations. Cellular integrity was confirmed by a trypan blue dye exclusion index of 93%, (3H) leucine incorporation, and ultrastructural analysis. Unit gravity sedimentation is an affective and rapid procedure for obtaining viable, homogeneous preparations of basal and mucous cells that may be used for in vitro studies of cellular proliferation, differentiation, and glycoprotein biosynthesis in respiratory mucous epithelia.
- Research Organization:
- Department of Obstetrics and Gynecology, University of Pennsylvania School of Medicine, Philadelphia
- OSTI ID:
- 5466999
- Journal Information:
- Am. Rev. Respir. Dis.; (United States), Journal Name: Am. Rev. Respir. Dis.; (United States) Vol. 124:6; ISSN ARRDA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AROMATICS
AZO COMPOUNDS
AZO DYES
CARBOXYLIC ACIDS
DYES
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
LEUCINE
MAMMALS
NAPHTHOLS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHENOLS
RABBITS
RESPIRATORY SYSTEM
RESPIRATORY TRACT CELLS
SEDIMENTATION
SEPARATION PROCESSES
SULFONIC ACIDS
TRACHEA
TRITIUM COMPOUNDS
TRYPAN BLUE
UPTAKE
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AROMATICS
AZO COMPOUNDS
AZO DYES
CARBOXYLIC ACIDS
DYES
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
LEUCINE
MAMMALS
NAPHTHOLS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHENOLS
RABBITS
RESPIRATORY SYSTEM
RESPIRATORY TRACT CELLS
SEDIMENTATION
SEPARATION PROCESSES
SULFONIC ACIDS
TRACHEA
TRITIUM COMPOUNDS
TRYPAN BLUE
UPTAKE
VERTEBRATES