Effect of calcium on sulfated mucous glycoprotein secretion in dog trachea

Journal Article · Fri Jan 01 04:00:00 EST 1982 · J. Appl. Physiol.: Respir., Environ. Exercise Physiol.; (United States)

OSTI ID:5428810

Martin, M G; Estep, J A; Zorn, J P

In this experiment utilizing dog trachea we determined the effect of calcium concentration in the bathing media on the kinetics of secretion of sulfate incorporated into mucous glycoprotein. The results showed that in eight tracheas the secretion rate of 269 +/- 65 pmol SO/sub 4/ . cm/sup -2/ . hr/sup -1/ (mean +/- SE) of tissues bathed in solution lacking calcium throughout the experiment was significantly less than the rate of 526 +/- 87 pmol SO/sub 4/ . cm/sup -2/ . hr/sup -1/ of tissues bathed in solution containing 1.9 mM Ca/sup 2 +/. When tissues bathed in 1.9 mM Ca/sup 2 +/ were incubated with /sup 35/SO/sub 4/ to a constant secretion rate and then placed in 0 mM Ca/sup 2 +/ solution, the secretion of incorporated sulfate was unchanged relative to tissues bathed in 1.9 mM Ca/sup 2 +/ throughout the experiment. Pool size of 727 +/- 150 pmol SO/sub 4//cm/sup 2/ in tissues incubated in 0 mM Ca/sup 2 +/ throughout the experiment was significantly less than the pool size of 1m,069 +/- 190 pmol SO/sub 4//cm/sup 2/ in 1.9 mM Ca/sup 2 +/ solutions. This study showed that either the uptake of sulfate and/or biosynthetic pool size of sulfate incorporated into mucous glycoproteins was decreased in solution lacking calcium. However, release of sulfate incorporated into mucous glycoprotein in the lumen was independent of calcium in the external bathing media.

• Research Organization: Univ. of Rochester, NY
• DOE Contract Number: AC02-76EV03490
• OSTI ID: 5428810
• Journal Information: J. Appl. Physiol.: Respir., Environ. Exercise Physiol.; (United States), Journal Name: J. Appl. Physiol.: Respir., Environ. Exercise Physiol.; (United States) Vol. 52:1; ISSN JARPD
• Country of Publication: United States
• Language: English

Related Subjects

560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALINE EARTH METAL COMPOUNDS
ALKALINE EARTH METALS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL EFFECTS
BODY
CALCIUM
CALCIUM COMPOUNDS
CARBOHYDRATES
DOGS
ELEMENTS
EPITHELIUM
GLUCOPROTEINS
MAMMALS
MEMBRANES
METABOLISM
METALS
MUCOUS MEMBRANES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
RESPIRATORY SYSTEM
RESPONSE MODIFYING FACTORS
SACCHARIDES
SECRETION
SULFATES
SULFUR COMPOUNDS
TISSUES
TRACHEA
VERTEBRATES