Nucleotide sequence of the tobacco (Nicotiana tabacum) anionic peroxidase gene
- Ohio State Univ., Columbus (United States)
Peroxidases have been implicated in numerous physiological processes including lignification (Grisebach, 1981), wound-healing (Espelie et al., 1986), phenol oxidation (Lagrimini, 1991), pathogen defense (Ye et al., 1990), and the regulation of cell elongation through the formation of interchain covalent bonds between various cell wall polymers (Fry, 1986; Goldberg et al., 1986; Bradley et al., 1992). However, a complete description of peroxidase action in vivo is not available because of the vast number of potential substrates and the existence of multiple isoenzymes. The tobacco anionic peroxidase is one of the better-characterized isoenzymes. This enzyme has been shown to oxidize a number of significant plant secondary compounds in vitro including cinnamyl alcohols, phenolic acids, and indole-3-acetic acid (Maeder, 1980; Lagrimini, 1991). A cDNA encoding the enzyme has been obtained, and this enzyme was shown to be expressed at the highest levels in lignifying tissues (xylem and tracheary elements) and also in epidermal tissue (Lagrimini et al., 1987). It was shown at this time that there were four distinct copies of the anionic peroxidase gene in tobacco (Nicotiana tabacum). A tobacco genomic DNA library was constructed in the [lambda]-phase EMBL3, from which two unique peroxidase genes were sequenced. One of these clones, [lambda]POD1, was designated as a pseudogene when the exonic sequences were found to differ from the cDNA sequences by 1%, and several frame shifts in the coding sequences indicated a dysfunctional gene (the authors' unpublished results). The other clone, [lambda]POD3, described in this manuscript, was designated as the functional tobacco anionic peroxidase gene because of 100% homology with the cDNA. Significant structural elements include an AS-2 box indicated in shoot-specific expression (Lam and Chua, 1989), a TATA box, and two intervening sequences. 10 refs., 1 tab.
- DOE Contract Number:
- FG02-89ER14004
- OSTI ID:
- 6448412
- Journal Information:
- Plant Physiology; (United States), Journal Name: Plant Physiology; (United States) Vol. 101:3; ISSN 0032-0889; ISSN PLPHAY
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CELL CONSTITUENTS
CELL WALL
CONTROL
DNA SEQUENCING
ELONGATION
ENZYMES
GENES
GENETIC CONTROL
ISOENZYMES
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NICOTIANA
NUCLEOTIDES
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEROXIDASES
PEST CONTROL
PHYSIOLOGY
PLANTS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
TOBACCO