The molecular characterization of the lignin-forming peroxidase. Progress summary report, April 1, 1989--March 31, 1992

Technical Report · Wed Apr 01 04:00:00 EST 1992

DOI:https://doi.org/10.2172/10135331· OSTI ID:10135331

Lagrimini, L M

This laboratory is committed to understanding the function of plant peroxidases via a multi-disciplinary approach. We have chosen the lignin-forming peroxidase from tobacco as the first isoenzyme to be subjected to this comprehensive approach. The goals which were set out upon the initiation of this project were as follows: (1) utilize a cDNA clone to the tobacco anionic peroxidase to generate transgenic plants which either over-produced this isoenzyme or specifically under-produced this isoenzyme via antisense RNA, (2) describe any phenotypic changes resulting from altered peroxidase expression, (3) perform morphological, physiological, and biochemical analysis of the above mentioned plants to help in determining the in planta function for this enzyme, and (4) clone and characterize the gene for the tobacco anionic peroxidase. A summary of progress thus far which includes both published and unpublished work will be presented in three sections: generation and characterization of transgenic plants, description of phenotypes, and biochemical and physiological analysis of peroxidase function, and cloning and characterization of the tobacco anionic peroxidase gene.

• Research Organization: Ohio State Univ., Columbus, OH (United States). Dept. of Horticulture
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: FG02-89ER14004
• OSTI ID: 10135331
• Report Number(s): DOE/ER/14004--3; ON: DE92010795
• Country of Publication: United States
• Language: English

Related Subjects

550200
550400
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMISTRY
CLONING
DNA
DNA SEQUENCING
ENZYME ACTIVITY
FERTILITY
GENETICS
LIGNIN
MORPHOLOGY
PEROXIDASES
PHENOTYPE
PROGRESS REPORT
TOBACCO