The molecular characterization of the lignin-forming peroxidase

Technical Report · Wed Jan 01 04:00:00 EST 1992

DOI:https://doi.org/10.2172/5445343· OSTI ID:5445343

Lagrimini, L M

This laboratory is committed to understanding the function of plant peroxidases via a multi-disciplinary approach. We have chosen the lignin-forming peroxidase from tobacco as the first isoenzyme to be subjected to this comprehensive approach. The goals which were set out upon the initiation of this project were as follows: (1) utilize a cDNA clone to the tobacco anionic peroxidase to generate transgenic plants which either over-produced this isoenzyme or specifically under-produced this isoenzyme via antisense RNA, (2) describe any phenotypic changes resulting from altered peroxidase expression, (3) perform morphological, physiological, and biochemical analysis of the above mentioned plants to help in determining the in planta function for this enzyme, and (4) clone and characterize the gene for the tobacco anionic peroxidase. A summary of progress thus far which includes both published and unpublished work will be presented in three sections: generation and characterization of transgenic plants, description of phenotypes, and biochemical and physiological analysis of peroxidase function, and cloning and characterization of the tobacco anionic peroxidase gene.

• Research Organization: Ohio State Univ., Columbus, OH (United States). Dept. of Horticulture
• Sponsoring Organization: DOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG02-89ER14004
• OSTI ID: 5445343
• Report Number(s): DOE/ER/14004-3; ON: DE92010795
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CARBOHYDRATES
CLONING
DNA
DNA SEQUENCING
DOCUMENT TYPES
ENZYME ACTIVITY
ENZYMES
FERTILITY
LIGNIN
MORPHOLOGY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEROXIDASES
PHENOTYPE
POLYSACCHARIDES
PROGRESS REPORT
PROTEINS
SACCHARIDES
STRUCTURAL CHEMICAL ANALYSIS
TOBACCO