The human T-cell receptor TCRAC/TCRDC (C[alpha]/C[delta]) region: Organization, sequence, and evolution of 97. 6 kb on DNA

Journal Article · Tue Feb 01 00:00:00 EST 1994 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1994.1097· OSTI ID:6443687

Koop, B F ^[1]; Rowen, L; Wang, Kai ^[2]; Lam Kuo, Chia; Howard, S; Shan, Wei; Deshpande, P ^[3]; Seto, D ^[4]; Lenstra, J A ^[5]; Hood, L ^[6]

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1. Univ. of Victoria, British Columbia (Canada)
2. Univ. of Washington, Seattle, WA (United States)
3. California Institute of Technology, Pasadena, CA (United States)
4. National Institutes of Health, Bethesda, MD (United States)
5. Dept. of Infectious Diseases and Immunology, Utrecht (Netherlands)
6. Univ. of Washington, Seattle, WA (United States) California Institute of Technology, Pasadena, CA (United States)

The authors sequenced and analyzed 97.6 kb of new DNA sequence containing the human TCRAC (C[alpha]) and TCRDC (C[delta]) genes as well as the TCRDV3 (V[delta]3) and 61 different TCRAJ (J[alpha]) gene segments and compared its organization and structure to the previously described mouse T-cell receptor TCRAC/TCRDC (C[alpha]/C[delta]) region. A comprehensive nomenclature, consistent with the IUIS nomenclature committee recommendations, for both human and mouse TCRAJ gene segments is presented. In the human sequence, they identified 20 new TCRAJ gene segments and obtained the germline sequence for 23 additional TCRAJ gene segments known from cDNA clones. Using the sequence data obtained from the human TCRAC/TCRDC region, they have extended a polymerase chain reaction-based assay to test for the expression of the individual TCRAJ gene segments. At least five TCRAJ pseudogene segments were identified by sequence criteria. Like the murine TCRAC/TCRDC sequence, this sequence contains a high level of coding sequence, with over 6.6% of the total sequence being transcribed. Comparison of the human sequence with the previously reported mouse DNA sequence reveals homologous counterparts for the variable and joining (J) gene segments and both constant genes. Eleven new J pseudogene segments have been identified in the mouse TCRAC/TCRDC sequence through the use of human and mouse sequence comparisons. In terms of structure and organization, this region of the human and mouse genome appears to be remarkably conserved. 38 refs., 5 figs., 1 tab.

• OSTI ID: 6443687
• Journal Information: Genomics; (United States), Vol. 19:3; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
DNA SEQUENCING
RECEPTORS
BIOLOGICAL EVOLUTION
LYMPHOCYTES
MAN
MICE
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
LEUKOCYTES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
RODENTS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550400* - Genetics