Genes in a 220-kb region spanning the TNF cluster in human MHC

Journal Article · Mon Jan 15 04:00:00 EST 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0034· OSTI ID:501819

Nalabolu, S R; Nallur, G; Weissman, S M ^[1]

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1. Yale Univ. School of Medicine, New Haven, CT (United States); and others

A search for new genes was performed in a 220-kb region around the tumor necrosis factor gene cluster in the human central major histocompatibility complex region using a cDNA hybridization and selection method. In addition to the seven known genes in this region, we identified a new gene that is preferentially expressed in spleen. We also identified two pseudogenes that have high degrees of homology to cytokeratin and cyclophillin, respectively. Expressed sequences for a human homologue of the mouse B144 gene were also found in the current analysis. RT-PCR analysis showed that B144 is expressed in spleen, in thymus, and prominently in the macrophage cell line, U937. We also independently identified the BAT1 gene to be the well-conserved homologue of a previously described rat liver nuclear protein. 53 refs., 6 figs., 1 tab.

• OSTI ID: 501819
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 31; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AMINO ACID SEQUENCE
ANTIGENS
CONTIGS
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
ELECTROPHORESIS
GENE REGULATION
GENES
GENETIC MAPPING
HEREDITARY DISEASES
HISTOCOMPATIBILITY COMPLEX
HUMAN CHROMOSOME 6
IMMUNE SYSTEM DISEASES
MAN
POLYMERASE CHAIN REACTION
SPLICING
STRUCTURE-ACTIVITY RELATIONSHIPS
TISSUE DISTRIBUTION