Human major histocompatibility complex contains a minimum of 19 genes between the complement cluster and HLA-B

Journal Article · Wed Nov 01 04:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.22.8955· OSTI ID:6855031

Spies, T; Bresnahan, M; Strominger, J L ^[1]

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1. Harvard Univ., Cambridge, MA (USA)

A 600-kilobase (kb) DNA segment from the human major histocompatibility complex (MHC) class III region was isolated by extension of a previous 435-kb chromosome walk. The contiguous series of cloned overlapping cosmids contains the entire 555-kb interval between C2 in the complement gene cluster and HLA-B. This region is known to encode the tumor necrosis factors (TNFs) {alpha} and {beta}, B144, and the major heat shock protein HSP70. Moreover, a cluster of genes, BAT1-BAT5 (HLA-B-associated transcripts) have been localized in the vicinity of the genes for TNF{alpha} and TNF{beta}. An additional four genes were identified by isolation of corresponding cDNA clones with cosmid DNA probes. These genes for BAT6-BAT9 were mapped near the gene for C2 within a 120-kb region that includes a HSP70 gene pair. These results, together with complementary data from a similar recent study, indicated the presence of a minimum of 19 genes within the C2-HLA-B interval of the MHC class III region. Although the functional properties of most of these genes are yet unknown, they may be involved in some aspects of immunity. This idea is supported by the genetic mapping of the hematopoietic histocompatibility locus-1 (Hh-1) in recombinant mice between TNF{alpha} and H-2S, which is homologous to the complement gene cluster in humans.

• OSTI ID: 6855031
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA) Vol. 86:22; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
AUTORADIOGRAPHY
CHROMOSOMES
CLONING
DNA
DNA HYBRIDIZATION
DNA-CLONING
GENE RECOMBINATION
GENES
GENETIC MAPPING
HELA CELLS
HYBRIDIZATION
MAMMALS
MAN
MAPPING
MESSENGER-RNA
MICE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
RECOMBINANT DNA
RNA
RODENTS
VERTEBRATES