Organization, sequence, and function of 34. 5 kb of genomic DNA encompassing several murine T-cell receptor [alpha]/[delta] variable gene segments

Journal Article · Tue Mar 15 04:00:00 EST 1994 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1994.1162· OSTI ID:6821706

Seto, D; Koop, B F; Deshpande, P; Howard, S; Seto, J; Wilk, E; Wang, K; Hood, L ^[1]

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1. California Institute of Tech., Pasadena, CA (United States)

A cosmid-bearing murine genomic DNA, encompassing several T-cell receptor variable [alpha]/[delta] gene segments, has been sequenced using shotgun DNA cloning methodology coupled with fluorescence-based high-throughput DNA sequencing technology. This region, spanning 34.5 kb, contains a pseudogene V[alpha] gene segment ([Psi]V[alpha]16.1), a V[delta] gene segment (V[delta]2), and a variable gene segment that has been reported to be expressed with both C[alpha] (V[alpha]6) and C[delta] (V[delta]3). Therefore, this cosmid is the ideal vehicle for examining the possible control sequences that may be involved in determining whether a V gene segment associates with either C[alpha] or C[delta]. Polymerase chain reaction experiments demonstrate that the two functional variable gene segments (V[delta] and V[alpha]/[delta]) are expressed individually with both C[alpha] and C[delta] genes as mRNA, indicating a permissiveness in their expression patterns. In addition, a variety of genomic sequence-related features have been identified. 52 refs., 4 figs.

• DOE Contract Number: FG03-91ER61182
• OSTI ID: 6821706
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 20:2; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
DNA SEQUENCING
GENES
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
PROTEINS
RECEPTORS
STRUCTURAL CHEMICAL ANALYSIS