Proliferative kinetics of large and small intraepithelial lymphocytes in the small intestine of the mouse

Journal Article · Mon Mar 01 04:00:00 EST 1976 · Am. J. Anat.; (United States)

DOI:https://doi.org/10.1002/aja.1001450307· OSTI ID:6434170

Roepke, C ^[1]; Everett, N B

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1. Univ. of Washington, Seattle

The proliferative kinetics of the intraepithelial lymphocytes (IL) of the mouse intestine have been evaluated. By inducing mitotic arrest it was found that large IL--constituting about 50% of the IL--showed a mitotic rate of 2.3. Autoradiographic results obtained after two different schedules of /sup 3/H-thymidine injections showed that 30% of the large IL were in DNA synthesis, and that the large IL were renewed at a rate comparable to that of blast cells from Peyer's patches, mesenteric lymph nodes and thoracic duct lymph. The small IL were renewed very rapidly compared to small lymphocytes of peripheral lymphoid tissues, although small lymphocytes with lifespans of several weeks were also present in the epithelial sheet. By the use of intestinal perfusion, in vivo, it was estimated that the loss of lymphocytes from intestinal villi into the lumen of the gut was negligible, and it is concluded that the most probable kinetic model for the majority of IL is: B and T lymphoblasts invade the epithelium and undergo mitosis. B lymphoblasts give rise predominantly to plasma cells, and T lymphoblasts give rise to small lymphocytes--probably long-lived--which reenter the circulation.

• OSTI ID: 6434170
• Journal Information: Am. J. Anat.; (United States), Journal Name: Am. J. Anat.; (United States) Vol. 145:3; ISSN AJANA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DIGESTIVE SYSTEM
GASTROINTESTINAL TRACT
INTESTINES
KINETICS
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MICE
ORGANS
RODENTS
SMALL INTESTINE
SOMATIC CELLS
VERTEBRATES