Intraepithelial lymphocytes express junctional molecules in murine small intestine

Journal Article · Fri Jun 17 04:00:00 EDT 2005 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/j.bbrc.2005.04.025· OSTI ID:20710794

Inagaki-Ohara, Kyoko ^[1]; Sawaguchi, Akira ^[2]; Suganuma, Tatsuo ^[2]; Matsuzaki, Goro ^[3]; Nawa, Yukifumi ^[1]

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1. Parasitic Diseases Unit, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki 889-1692 (Japan)
2. Department of Anatomy, Faculty of Medicine, University of Miyazaki, Kiyotake, Miyazaki 889-1692 (Japan)
3. Molecular Microbiology Group, Center of Molecular Biosciences, University of the Ryukyus, Senbaru, Nishihara, Okinawa 903-0213 (Japan)

Intestinal intraepithelial lymphocytes (IEL) that reside at basolateral site regulate the proliferation and differentiation of epithelial cells (EC) for providing a first line of host defense in intestine. However, it remains unknown how IEL interact and communicate with EC. Here, we show that IEL express junctional molecules like EC. We identified mRNA expression of the junctional molecules in IEL such as zonula occludens (ZO)-1, occludin and junctional adhesion molecule (JAM) (tight junction), {beta}-catenin and E-cadherin (adherens junction), and connexin26 (gap junction). IEL constitutively expressed occludin and E-cadherin at protein level, while other T cells in the thymus, spleen, liver, mesenteric lymph node, and Peyer's patches did not. {gamma}{delta} IEL showed higher level of these expressions than {alpha}{beta} IEL. The expression of occludin was augmented by anti-CD3 Ab stimulation. These results suggest the possibility of a novel role of IEL concerning epithelial barrier and communication between IEL and EC.

• OSTI ID: 20710794
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 4 Vol. 331; ISSN BBRCA9; ISSN 0006-291X
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL PROLIFERATION
LIVER
LYMPH NODES
LYMPHOCYTES
PROTEINS
SMALL INTESTINE
SPLEEN
THYMUS